Reference values of amino acids, acylcarnitines and succinylacetone by tandem mass spectrometry for use in newborn screening in southwest Colombia
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| Format: | Artículo científico |
| Language: | en |
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Universidad del Valle
2017
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| author | Nora Céspedes |
| author_facet | Nora Céspedes |
| contents | Reference values of amino acids, acylcarnitines and succinylacetone by tandem mass spectrometry for use in newborn screening in southwest Colombia Nora Céspedes Angela Valencia Carlos Alberto Echeverry Maria Isabel Arce-Plata Cristóbal Colón Daisy E Castiñeiras Paula Margarita Hurtado Jose Angel Cocho Sócrates Herrera Myriam Arévalo-Herrera Medicina Tandem amino acids inborn errors acylcarnitines Succinylacetone Introduction: Inborn errors of metabolism (IEM) represent an important public health problem due to current diagnosis and treatment limitations, poor life quality of affected patients, and consequent untimely child death. In contrast to classical methods, tandem mass spectrometry (MS/MS) has allowed simultaneous evaluation of multiple metabolites associated with IEM offering higher sensitivity, low false positive rates and high throughput.Aims: Determine concentration levels for amino acids and acylcarnitines in blood of newborns from Colombia, to establish reference values for further use in diagnosis of IEM.Methods: Implementation of a method to determine amino acids, acylcarnitines and succinylacetone in newborn dried blood spots using MS/MS, and its application in a cross-sectional study conducted in 891 healthy neonates from Cali and Quibdo cities is described. Results: fifty-seven analytes that allow the diagnosis of more than 40 different pathologies were tested. The method showed to be linear, precise and accurate. Healthy neonates 1-18 days of age were included, 523 from Cali and 368 from Quibdo; 52% male and 48% female. Age-related differences on the concentration levels of amino acids and acylcarnitines were observed whereas no significant differences by gender were found. Conclusion: The study has contributed to reveal the usual concentration levels of amino acids, acylcarnitines and succinylacetone that could be used as reference for the establishment of a newborn metabolic screening program in Colombia. 2017 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28353727002 https://www.redalyc.org/journal/283/28353727002/ https://www.redalyc.org/journal/283/28353727002/html/ https://www.redalyc.org/journal/283/28353727002/28353727002.epub https://www.redalyc.org/journal/283/28353727002/movil 10.25100/cm.v48i3.2180 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.3 Vol.48 |
| format | Artículo científico |
| id | redalyc_28353727002 |
| institution | Redalyc |
| language | en |
| publishDate | 2017 |
| publisher | Universidad del Valle |
| spellingShingle | Reference values of amino acids, acylcarnitines and succinylacetone by tandem mass spectrometry for use in newborn screening in southwest Colombia Nora Céspedes Medicina Tandem amino acids inborn errors acylcarnitines Succinylacetone Reference values of amino acids, acylcarnitines and succinylacetone by tandem mass spectrometry for use in newborn screening in southwest Colombia Nora Céspedes Angela Valencia Carlos Alberto Echeverry Maria Isabel Arce-Plata Cristóbal Colón Daisy E Castiñeiras Paula Margarita Hurtado Jose Angel Cocho Sócrates Herrera Myriam Arévalo-Herrera Medicina Tandem amino acids inborn errors acylcarnitines Succinylacetone Introduction: Inborn errors of metabolism (IEM) represent an important public health problem due to current diagnosis and treatment limitations, poor life quality of affected patients, and consequent untimely child death. In contrast to classical methods, tandem mass spectrometry (MS/MS) has allowed simultaneous evaluation of multiple metabolites associated with IEM offering higher sensitivity, low false positive rates and high throughput.Aims: Determine concentration levels for amino acids and acylcarnitines in blood of newborns from Colombia, to establish reference values for further use in diagnosis of IEM.Methods: Implementation of a method to determine amino acids, acylcarnitines and succinylacetone in newborn dried blood spots using MS/MS, and its application in a cross-sectional study conducted in 891 healthy neonates from Cali and Quibdo cities is described. Results: fifty-seven analytes that allow the diagnosis of more than 40 different pathologies were tested. The method showed to be linear, precise and accurate. Healthy neonates 1-18 days of age were included, 523 from Cali and 368 from Quibdo; 52% male and 48% female. Age-related differences on the concentration levels of amino acids and acylcarnitines were observed whereas no significant differences by gender were found. Conclusion: The study has contributed to reveal the usual concentration levels of amino acids, acylcarnitines and succinylacetone that could be used as reference for the establishment of a newborn metabolic screening program in Colombia. 2017 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28353727002 https://www.redalyc.org/journal/283/28353727002/ https://www.redalyc.org/journal/283/28353727002/html/ https://www.redalyc.org/journal/283/28353727002/28353727002.epub https://www.redalyc.org/journal/283/28353727002/movil 10.25100/cm.v48i3.2180 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.3 Vol.48 |
| title | Reference values of amino acids, acylcarnitines and succinylacetone by tandem mass spectrometry for use in newborn screening in southwest Colombia |
| topic | Medicina Tandem amino acids inborn errors acylcarnitines Succinylacetone |
| url | https://www.redalyc.org/articulo.oa?id=28353727002 https://www.redalyc.org/journal/283/28353727002/ https://www.redalyc.org/journal/283/28353727002/html/ https://www.redalyc.org/journal/283/28353727002/28353727002.epub https://www.redalyc.org/journal/283/28353727002/movil |