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| Lenguaje: | en |
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Universidad del Valle
2017
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| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=28354693003 https://www.redalyc.org/journal/283/28354693003/ https://www.redalyc.org/journal/283/28354693003/html/ https://www.redalyc.org/journal/283/28354693003/28354693003.epub https://www.redalyc.org/journal/283/28354693003/movil |
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- Real-time PCR assay for the diagnosis of pleural tuberculosis Martha Alejandra Casallas-Rivera Ana María Cárdenas Bernal Luis Fernando Giraldo-Cadavid Diago Enrique Prieto Sandra Paola Santander Medicina Real Ziehl Neelsen Diagnostic tests pleural effusion Introduction: The diagnosis of pleural tuberculosis requires an invasive and time-consuming reference method. Polymerase chain reaction (PCR) is rapid, but validation in pleural tuberculosis is still weak.Objective: To establish the operating characteristics of real-time polymerase chain reaction (RT-PCR) hybridization probes for the diagnosis of pleural tuberculosis.Methods: The validity of the RT-PCR hybridization probes was evaluated compared to a composite reference method by a cross-sectional study at the Hospital Universitario de la Samaritana. 40 adults with lymphocytic pleural effusion were included. Pleural tuberculosis was confirmed (in 9 patients) if the patient had at least one of three tests using the positive reference method: Ziehl-Neelsen or Mycobacterium tuberculosis culture in fluid or pleural tissue, or pleural biopsy with granulomas. Pleural tuberculosis was ruled out (in 31 patients) if all three tests were negative. The operating characteristics of the RT-PCR, using the Mid-P Exact Test, were determined using the OpenEpi 2.3 Software (2009).Results: The RT-PCR hybridization probes showed a sensitivity of 66.7% (95% CI: 33.2%-90.7%) and a specificity of 93.5% (95% CI: 80.3%-98.9%). The PPV was 75.0% (95% CI: 38.8%-95.6%) and a NPV of 90.6% (95% CI: 76.6%-97.6%). Two false positives were found for the test, one with pleural mesothelioma and the other with chronic pleuritis with mesothelial hyperplasia. Conclusions: The RT-PCR hybridization probes had good specificity and acceptable sensitivity, but a negative value cannot rule out pleural tuberculosis. 2017 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28354693003 https://www.redalyc.org/journal/283/28354693003/ https://www.redalyc.org/journal/283/28354693003/html/ https://www.redalyc.org/journal/283/28354693003/28354693003.epub https://www.redalyc.org/journal/283/28354693003/movil en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.2 Vol.48