Distribution of polymorphism rs693 of ApoB gene in a sample of Colombian Caribbeans.
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| Format: | Artículo científico |
| Langue: | en |
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Universidad del Valle
2019
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| _version_ | 1876453886856790016 |
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| author | Evelyn Mendoza-Torres |
| author_facet | Evelyn Mendoza-Torres |
| contents | Distribution of polymorphism rs693 of ApoB gene in a sample of Colombian Caribbeans. Evelyn Mendoza-Torres Nicole Samuel Pereira Sanandrés José Luis Villarreal Camacho Xilene Mendoza Sánchez César De La Espriella Pérez Lourdes Luz Varela Prieto Daniel Antonio Villanueva Torregrosa Medicina genotyping polymorphism lipid profile body mass index Introduction: Several studies have reported that the single nucleotide polymorphism rs693 of Apo lipoprotein B gene is associated with high levels of plasma lipids and high body mass index, which are risk factors for cardiovascular diseases. The distribution of this single nucleotide polymorphism and its association with the phenotype depend on the genetic background of each population. Objective: To evaluate the distribution of single nucleotide polymorphism rs693 and its association with lipid profile and body mass index in a sample of Colombian Caribbeans.Methods: 108 non-related adult subjects of both gender were included in this study. Body mass index and lipid profile that included total cholesterol, triglycerides, Low Density Lipoprotein and High Density Lipoprotein were determined. The single nucleotide polymorphism rs693 was determined by Polymerase Chain Reaction/Restriction Fragment Length Polymorphism from genomic DNA followed by digestion with the restriction enzyme XbaI. The chi-square test was used to analyze the genotype distribution of rs693 and the genotype-phenotype association was evaluated through different inheritance model. Results: The genotype frequencies for single nucleotide polymorphism rs693 were CC (45.0%), TT (16.5%) and CT (38.5%). The allele frequencies were C (64.0%) and T (36.0%). The single nucleotide polymorphism was in Hardy-Weinberg equilibrium in the studied sample. No association of the single nucleotide polymorphism rs693 with lipid profile nor the body mass index was found (p >0.05).Conclusion: There is no significant association between single nucleotide polymorphism rs693 and body mass index nor lipid profile, in a sample of Colombian Caribbeans. 2019 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28362904003 https://www.redalyc.org/journal/283/28362904003/ https://www.redalyc.org/journal/283/28362904003/html/ https://www.redalyc.org/journal/283/28362904003/28362904003.epub https://www.redalyc.org/journal/283/28362904003/movil 10.25100/cm.v50i3.4048 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.3 Vol.50 |
| format | Artículo científico |
| id | redalyc_28362904003 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Universidad del Valle |
| spellingShingle | Distribution of polymorphism rs693 of ApoB gene in a sample of Colombian Caribbeans. Evelyn Mendoza-Torres Medicina genotyping polymorphism lipid profile body mass index Distribution of polymorphism rs693 of ApoB gene in a sample of Colombian Caribbeans. Evelyn Mendoza-Torres Nicole Samuel Pereira Sanandrés José Luis Villarreal Camacho Xilene Mendoza Sánchez César De La Espriella Pérez Lourdes Luz Varela Prieto Daniel Antonio Villanueva Torregrosa Medicina genotyping polymorphism lipid profile body mass index Introduction: Several studies have reported that the single nucleotide polymorphism rs693 of Apo lipoprotein B gene is associated with high levels of plasma lipids and high body mass index, which are risk factors for cardiovascular diseases. The distribution of this single nucleotide polymorphism and its association with the phenotype depend on the genetic background of each population. Objective: To evaluate the distribution of single nucleotide polymorphism rs693 and its association with lipid profile and body mass index in a sample of Colombian Caribbeans.Methods: 108 non-related adult subjects of both gender were included in this study. Body mass index and lipid profile that included total cholesterol, triglycerides, Low Density Lipoprotein and High Density Lipoprotein were determined. The single nucleotide polymorphism rs693 was determined by Polymerase Chain Reaction/Restriction Fragment Length Polymorphism from genomic DNA followed by digestion with the restriction enzyme XbaI. The chi-square test was used to analyze the genotype distribution of rs693 and the genotype-phenotype association was evaluated through different inheritance model. Results: The genotype frequencies for single nucleotide polymorphism rs693 were CC (45.0%), TT (16.5%) and CT (38.5%). The allele frequencies were C (64.0%) and T (36.0%). The single nucleotide polymorphism was in Hardy-Weinberg equilibrium in the studied sample. No association of the single nucleotide polymorphism rs693 with lipid profile nor the body mass index was found (p >0.05).Conclusion: There is no significant association between single nucleotide polymorphism rs693 and body mass index nor lipid profile, in a sample of Colombian Caribbeans. 2019 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28362904003 https://www.redalyc.org/journal/283/28362904003/ https://www.redalyc.org/journal/283/28362904003/html/ https://www.redalyc.org/journal/283/28362904003/28362904003.epub https://www.redalyc.org/journal/283/28362904003/movil 10.25100/cm.v50i3.4048 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.3 Vol.50 |
| title | Distribution of polymorphism rs693 of ApoB gene in a sample of Colombian Caribbeans. |
| topic | Medicina genotyping polymorphism lipid profile body mass index |
| url | https://www.redalyc.org/articulo.oa?id=28362904003 https://www.redalyc.org/journal/283/28362904003/ https://www.redalyc.org/journal/283/28362904003/html/ https://www.redalyc.org/journal/283/28362904003/28362904003.epub https://www.redalyc.org/journal/283/28362904003/movil |