BRCA1 and BRCA2 mutations in a sample of breast and ovarian cancer families from the Colombian pacific
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad del Valle
2019
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| author | Laura Cifuentes-C |
| author_facet | Laura Cifuentes-C |
| contents | BRCA1 and BRCA2 mutations in a sample of breast and ovarian cancer families from the Colombian pacific Laura Cifuentes-C Ana Lucia Rivera-Herrera Guillermo Barreto Medicina Germ BRCA1 BRCA2 Colombia line mutations Introduction: Breast cancer is the most common neoplasia of women from all over the world especially women from Colombia. 5%10% of all cases are caused by hereditary factors, 25% of those cases have mutations in the BRCA1/BRCA2 genes. Objective: The purpose of this study was to identify the mutations associated with the risk of familial breast and/or ovarian cancer in a population of Colombian pacific. Methods: 58 high-risk breast and/or ovarian cancer families and 20 controls were screened for germline mutations in BRCA1 and BRCA2, by Single Strand Conformation Polymorphism (SSCP) and sequencing. Results: Four families (6.9%) were found to carry BRCA1 mutations and eight families (13.8%) had mutations in BRCA2. In BRCA1, we found three Variants of Uncertain Significance (VUS), of which we concluded, using in silico tools, that c.8112C>G and c.3119G>A (p.Ser1040Asn) are probably deleterious, and c.3083G>A (p.Arg1028His) is probably neutral. In BRCA2, we found three variants of uncertain significance: two were previously described and one novel mutation. Using in silico analysis, we concluded that c.865A>G (p.Asn289Asp) and c.6427T>C (p.Ser2143Pro) are probably deleterious and c.125A>G (p.Tyr42Cys) is probably neutral. Only one of them has previously been reported in Colombia. We also identified 13 polymorphisms (4 in BRCA1 and 9 in BRCA2), two of them are associated with a moderate increase in breast cancer risk (BRCA2 c.1114A>C and c.875566T>C). Conclusion: According to our results, the Colombian pacific population presents diverse mutational spectrum for BRCA genes that differs from the findings in other regions in the country. 2019 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28362904004 https://www.redalyc.org/journal/283/28362904004/ https://www.redalyc.org/journal/283/28362904004/html/ https://www.redalyc.org/journal/283/28362904004/28362904004.epub https://www.redalyc.org/journal/283/28362904004/movil 10.25100/cm.v50i3.2385 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.3 Vol.50 |
| format | Artículo científico |
| id | redalyc_28362904004 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Universidad del Valle |
| spellingShingle | BRCA1 and BRCA2 mutations in a sample of breast and ovarian cancer families from the Colombian pacific Laura Cifuentes-C Medicina Germ BRCA1 BRCA2 Colombia line mutations BRCA1 and BRCA2 mutations in a sample of breast and ovarian cancer families from the Colombian pacific Laura Cifuentes-C Ana Lucia Rivera-Herrera Guillermo Barreto Medicina Germ BRCA1 BRCA2 Colombia line mutations Introduction: Breast cancer is the most common neoplasia of women from all over the world especially women from Colombia. 5%10% of all cases are caused by hereditary factors, 25% of those cases have mutations in the BRCA1/BRCA2 genes. Objective: The purpose of this study was to identify the mutations associated with the risk of familial breast and/or ovarian cancer in a population of Colombian pacific. Methods: 58 high-risk breast and/or ovarian cancer families and 20 controls were screened for germline mutations in BRCA1 and BRCA2, by Single Strand Conformation Polymorphism (SSCP) and sequencing. Results: Four families (6.9%) were found to carry BRCA1 mutations and eight families (13.8%) had mutations in BRCA2. In BRCA1, we found three Variants of Uncertain Significance (VUS), of which we concluded, using in silico tools, that c.8112C>G and c.3119G>A (p.Ser1040Asn) are probably deleterious, and c.3083G>A (p.Arg1028His) is probably neutral. In BRCA2, we found three variants of uncertain significance: two were previously described and one novel mutation. Using in silico analysis, we concluded that c.865A>G (p.Asn289Asp) and c.6427T>C (p.Ser2143Pro) are probably deleterious and c.125A>G (p.Tyr42Cys) is probably neutral. Only one of them has previously been reported in Colombia. We also identified 13 polymorphisms (4 in BRCA1 and 9 in BRCA2), two of them are associated with a moderate increase in breast cancer risk (BRCA2 c.1114A>C and c.875566T>C). Conclusion: According to our results, the Colombian pacific population presents diverse mutational spectrum for BRCA genes that differs from the findings in other regions in the country. 2019 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28362904004 https://www.redalyc.org/journal/283/28362904004/ https://www.redalyc.org/journal/283/28362904004/html/ https://www.redalyc.org/journal/283/28362904004/28362904004.epub https://www.redalyc.org/journal/283/28362904004/movil 10.25100/cm.v50i3.2385 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.3 Vol.50 |
| title | BRCA1 and BRCA2 mutations in a sample of breast and ovarian cancer families from the Colombian pacific |
| topic | Medicina Germ BRCA1 BRCA2 Colombia line mutations |
| url | https://www.redalyc.org/articulo.oa?id=28362904004 https://www.redalyc.org/journal/283/28362904004/ https://www.redalyc.org/journal/283/28362904004/html/ https://www.redalyc.org/journal/283/28362904004/28362904004.epub https://www.redalyc.org/journal/283/28362904004/movil |