Flow cytometry-detected changes in megaloblastic anemia secondary to cobalamin deficiency
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| Format: | Artículo científico |
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Universidad del Valle
2023
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| _version_ | 1876450066149933056 |
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| author | Vania Briones |
| author_facet | Vania Briones |
| contents | Flow cytometry-detected changes in megaloblastic anemia secondary to cobalamin deficiency Vania Briones Fernanda Figueroa Catalina Vidal Vicente Micolich Mauricio Chandia Medicina Hematopoiesis Erythroblasts flow cytometry bone marrow cell Megaloblastic anemia Introduction: Megaloblastic anemias secondary to Vitamin B12 deficiency are a group of pathologies produced by defective nuclear DNA synthesis.Objective: To describe the maturation alterations found in hematopoietic precursors of the bone marrow in a series of patients with megaloblastic anemia.Methods: Were included patients attended at the Regional Hospital of Concepción with bone marrow samples sent for the study of cytopenia by flow cytometry whose final diagnosis was megaloblastic anemia. The immunophenotype was performed with CD45, CD34, CD117, HLA-DR, markers of neutrophil (CD13, CD11b, CD10, CD16) and/or erythroblast (CD105, CD71, CD36) maturation.Results: From the flow cytometry laboratory database, 8 patients with megaloblastic anemia were identified, and myelodysplastic syndromes (n=9) and normal or reactive bone marrow (n=10) were used as controls. 44% were men, with a median age of 58 years. Megaloblastic anemia was associated with a higher proportion of size and complexity of erythroid and myeloid progenitors compared to lymphocytes compared to controls. The total percentage of erythroblasts and the proportion of CD34+ myeloid cells associated with erythroid lineage was higher in megaloblastic anemia, associated with a maturation arrest in the CD105+ precursor stage (69% vs 19% and 23%, p<0.001). The heterogeneity of CD36 and CD71 in megaloblastic anemia was similar to myelodysplastic syndromes.Conclusions: Megaloblastic anemia produces a heterogeneous involvement of hematopoiesis, characterized by a greater size and cellular complexity of precursors of the neutrophil and erythroid series and a maturation arrest of the erythroblasts. 2023 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28375744002 https://www.redalyc.org/journal/283/28375744002/ https://www.redalyc.org/journal/283/28375744002/html/ https://www.redalyc.org/journal/283/28375744002/28375744002.epub https://www.redalyc.org/journal/283/28375744002/movil http://doi.org/10.25100/cm.v54i2.5494 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.2 Vol.54 |
| format | Artículo científico |
| id | redalyc_28375744002 |
| institution | Redalyc |
| language | en |
| publishDate | 2023 |
| publisher | Universidad del Valle |
| spellingShingle | Flow cytometry-detected changes in megaloblastic anemia secondary to cobalamin deficiency Vania Briones Medicina Hematopoiesis Erythroblasts flow cytometry bone marrow cell Megaloblastic anemia Flow cytometry-detected changes in megaloblastic anemia secondary to cobalamin deficiency Vania Briones Fernanda Figueroa Catalina Vidal Vicente Micolich Mauricio Chandia Medicina Hematopoiesis Erythroblasts flow cytometry bone marrow cell Megaloblastic anemia Introduction: Megaloblastic anemias secondary to Vitamin B12 deficiency are a group of pathologies produced by defective nuclear DNA synthesis.Objective: To describe the maturation alterations found in hematopoietic precursors of the bone marrow in a series of patients with megaloblastic anemia.Methods: Were included patients attended at the Regional Hospital of Concepción with bone marrow samples sent for the study of cytopenia by flow cytometry whose final diagnosis was megaloblastic anemia. The immunophenotype was performed with CD45, CD34, CD117, HLA-DR, markers of neutrophil (CD13, CD11b, CD10, CD16) and/or erythroblast (CD105, CD71, CD36) maturation.Results: From the flow cytometry laboratory database, 8 patients with megaloblastic anemia were identified, and myelodysplastic syndromes (n=9) and normal or reactive bone marrow (n=10) were used as controls. 44% were men, with a median age of 58 years. Megaloblastic anemia was associated with a higher proportion of size and complexity of erythroid and myeloid progenitors compared to lymphocytes compared to controls. The total percentage of erythroblasts and the proportion of CD34+ myeloid cells associated with erythroid lineage was higher in megaloblastic anemia, associated with a maturation arrest in the CD105+ precursor stage (69% vs 19% and 23%, p<0.001). The heterogeneity of CD36 and CD71 in megaloblastic anemia was similar to myelodysplastic syndromes.Conclusions: Megaloblastic anemia produces a heterogeneous involvement of hematopoiesis, characterized by a greater size and cellular complexity of precursors of the neutrophil and erythroid series and a maturation arrest of the erythroblasts. 2023 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28375744002 https://www.redalyc.org/journal/283/28375744002/ https://www.redalyc.org/journal/283/28375744002/html/ https://www.redalyc.org/journal/283/28375744002/28375744002.epub https://www.redalyc.org/journal/283/28375744002/movil http://doi.org/10.25100/cm.v54i2.5494 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.2 Vol.54 |
| title | Flow cytometry-detected changes in megaloblastic anemia secondary to cobalamin deficiency |
| topic | Medicina Hematopoiesis Erythroblasts flow cytometry bone marrow cell Megaloblastic anemia |
| url | https://www.redalyc.org/articulo.oa?id=28375744002 https://www.redalyc.org/journal/283/28375744002/ https://www.redalyc.org/journal/283/28375744002/html/ https://www.redalyc.org/journal/283/28375744002/28375744002.epub https://www.redalyc.org/journal/283/28375744002/movil http://doi.org/10.25100/cm.v54i2.5494 |