The ber pathway genes and pon1 polymorphism: influence on dna damage in agriculture-exposed workers
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad del Bío Bío
2006
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| _version_ | 1876431555588521984 |
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| author | Paula Rohr |
| author_facet | Paula Rohr |
| contents | The ber pathway genes and pon1 polymorphism: influence on dna damage in agriculture-exposed workers Paula Rohr João Antônio Pegas Henriques Kátia Kvitko Juliana da Silva Bernardo Erdtmann Multidisciplinarias (Ciencias Sociales) NER PON Polymorphisms MN and Comet Assay Occupational exposure Pesticide exposure represents a potential risk for human health. Farmers from Rio Grande do Sul (the southernmoststate of Brazil) use different kinds of such substances and are therefore constantly exposed to thosechemical compounds. The aim of this study was to evaluate the influence of polymorphisms in PON1 and intwo BER genes (XRCC1 and OGG1 polymorphisms) in cell damage, analyzing a group of pesticide-exposedfarmers. Genotyping of the three polymorphisms was performed by PCR/RFLP. DNA damage was evaluatedby Comet assay (ID) and Micronuclei test (MN) in 107 pesticide-exposed workers and in 73 nonexposedpeople. Statistically significant differences between cases and controls, considering data from ID,MN and genotyping analysis, were tested by the non-parametric Mann-Whitney U test. PON polymorphismsshowed influence in cell damage in the pesticide-exposed group, considering the frequency of MN:homozygotes Gln/Gln presented a higher incidence of MN when compared to Arg/-(p<0.05). Control individualswith XRCC1 194 Arg/Trp presented better results considering ID and MN (p<0.05). Heterozygoteindividuals for OGG1 326 Ser/Cys showed lower incidence of MN (p<0.05). The results from the PON andXRCC1 genotypes combined showed an effective repair for individuals Gln/Gln + Arg /Trp (p< 0.05). ThePON and OGG1 genotypes combined showed an effective repair for individuals Gln/Arg + Cys/- (p=0.01).Our results demonstrated that the metabolizing gene PON and the BER genes may have an influence onexposure to pesticides. Combinations of metabolizing and repair genotypes modulate the final result ofincorporation of DNA damage after exposure. These results demonstrate the importance of consideringtogether metabolizing and repair pathways on the individual susceptibility to incorporate DNA damage afterexposure to xenobiotics. 2006 artículo científico 0717-196X https://www.redalyc.org/articulo.oa?id=29915208 en http://www.redalyc.org/revista.oa?id=299 Theoria application/pdf Universidad del Bío Bío Theoria (Chile) Num.2 Vol.15 |
| format | Artículo científico |
| id | redalyc_29915208 |
| institution | Redalyc |
| language | en |
| publishDate | 2006 |
| publisher | Universidad del Bío Bío |
| spellingShingle | The ber pathway genes and pon1 polymorphism: influence on dna damage in agriculture-exposed workers Paula Rohr Multidisciplinarias (Ciencias Sociales) NER PON Polymorphisms MN and Comet Assay Occupational exposure The ber pathway genes and pon1 polymorphism: influence on dna damage in agriculture-exposed workers Paula Rohr João Antônio Pegas Henriques Kátia Kvitko Juliana da Silva Bernardo Erdtmann Multidisciplinarias (Ciencias Sociales) NER PON Polymorphisms MN and Comet Assay Occupational exposure Pesticide exposure represents a potential risk for human health. Farmers from Rio Grande do Sul (the southernmoststate of Brazil) use different kinds of such substances and are therefore constantly exposed to thosechemical compounds. The aim of this study was to evaluate the influence of polymorphisms in PON1 and intwo BER genes (XRCC1 and OGG1 polymorphisms) in cell damage, analyzing a group of pesticide-exposedfarmers. Genotyping of the three polymorphisms was performed by PCR/RFLP. DNA damage was evaluatedby Comet assay (ID) and Micronuclei test (MN) in 107 pesticide-exposed workers and in 73 nonexposedpeople. Statistically significant differences between cases and controls, considering data from ID,MN and genotyping analysis, were tested by the non-parametric Mann-Whitney U test. PON polymorphismsshowed influence in cell damage in the pesticide-exposed group, considering the frequency of MN:homozygotes Gln/Gln presented a higher incidence of MN when compared to Arg/-(p<0.05). Control individualswith XRCC1 194 Arg/Trp presented better results considering ID and MN (p<0.05). Heterozygoteindividuals for OGG1 326 Ser/Cys showed lower incidence of MN (p<0.05). The results from the PON andXRCC1 genotypes combined showed an effective repair for individuals Gln/Gln + Arg /Trp (p< 0.05). ThePON and OGG1 genotypes combined showed an effective repair for individuals Gln/Arg + Cys/- (p=0.01).Our results demonstrated that the metabolizing gene PON and the BER genes may have an influence onexposure to pesticides. Combinations of metabolizing and repair genotypes modulate the final result ofincorporation of DNA damage after exposure. These results demonstrate the importance of consideringtogether metabolizing and repair pathways on the individual susceptibility to incorporate DNA damage afterexposure to xenobiotics. 2006 artículo científico 0717-196X https://www.redalyc.org/articulo.oa?id=29915208 en http://www.redalyc.org/revista.oa?id=299 Theoria application/pdf Universidad del Bío Bío Theoria (Chile) Num.2 Vol.15 |
| title | The ber pathway genes and pon1 polymorphism: influence on dna damage in agriculture-exposed workers |
| topic | Multidisciplinarias (Ciencias Sociales) NER PON Polymorphisms MN and Comet Assay Occupational exposure |
| url | https://www.redalyc.org/articulo.oa?id=29915208 |