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| Natura: | Artículo científico |
| Lingua: | en |
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Universidade Estadual de Maringá
2012
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=303026475008 |
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| _version_ | 1866815958545334272 |
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| author | Kerlley Cristina de Assis |
| author_facet | Kerlley Cristina de Assis |
| contents | In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume Kerlley Cristina de Assis Flávia Dionísio Pereira Juliana Silva Rodrigues Cabral Fabiano Guimarães Silva José Waldemar Silva Silvia Correa dos Santos Agrociencias cashew cashew tissue culture tissue culture in vitro propagation Anacardium othonianum Rizz. is a medicinal plant species that is native to the Brazilian savannah. Adult plants are different from other genus members in this ecosystem due to their size, and efforts to locate the plants may lead to their extraction from the savannah and, frequently, plant death. Micropropagation has played a significant role in the propagation and preservation of specimens of several plant species; therefore, this study evaluated the effect of various salt concentrations and culture medium volumes on the in vitro cultivation of A. othonianum Rizz. Trial (I) evaluated two culture media (MS and WPM) and three salt concentrations (100, 50 and 25%) in a completely randomized design. Trial (II) evaluated two culture media and salt concentrations, MS (50%) and WPM (100%), and five medium volumes (10, 15, 20, 25 and 30 mL per test tube) as a 2 x 5 factorial in a completely randomized design. After 30 and 60 days of growth, the percentage of plantlet survival, average plantlet and leaf lengths and the average number of leaves and buds per explant were evaluated. The MS (50 and 25%) media and WPM (100 and 50%) media were the most effective for plantlet regeneration. The best responses were observed in 15- and 25-mL volumes of the MS (50%) medium. Therefore, the use of a 15-mL volume is suggested for greater medium economy. 2012 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303026475008 10.4025/actasciagron.v34i1.10984 en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Num.1 Vol.34 |
| format | Artículo científico |
| id | redalyc_303026475008 |
| language | en |
| publishDate | 2012 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume Kerlley Cristina de Assis Agrociencias cashew cashew tissue culture tissue culture in vitro propagation In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume Kerlley Cristina de Assis Flávia Dionísio Pereira Juliana Silva Rodrigues Cabral Fabiano Guimarães Silva José Waldemar Silva Silvia Correa dos Santos Agrociencias cashew cashew tissue culture tissue culture in vitro propagation Anacardium othonianum Rizz. is a medicinal plant species that is native to the Brazilian savannah. Adult plants are different from other genus members in this ecosystem due to their size, and efforts to locate the plants may lead to their extraction from the savannah and, frequently, plant death. Micropropagation has played a significant role in the propagation and preservation of specimens of several plant species; therefore, this study evaluated the effect of various salt concentrations and culture medium volumes on the in vitro cultivation of A. othonianum Rizz. Trial (I) evaluated two culture media (MS and WPM) and three salt concentrations (100, 50 and 25%) in a completely randomized design. Trial (II) evaluated two culture media and salt concentrations, MS (50%) and WPM (100%), and five medium volumes (10, 15, 20, 25 and 30 mL per test tube) as a 2 x 5 factorial in a completely randomized design. After 30 and 60 days of growth, the percentage of plantlet survival, average plantlet and leaf lengths and the average number of leaves and buds per explant were evaluated. The MS (50 and 25%) media and WPM (100 and 50%) media were the most effective for plantlet regeneration. The best responses were observed in 15- and 25-mL volumes of the MS (50%) medium. Therefore, the use of a 15-mL volume is suggested for greater medium economy. 2012 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303026475008 10.4025/actasciagron.v34i1.10984 en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Num.1 Vol.34 |
| title | In vitro cultivation of Anacardium othonianum Rizz.: effects of salt concentration and culture medium volume |
| topic | Agrociencias cashew cashew tissue culture tissue culture in vitro propagation |
| url | https://www.redalyc.org/articulo.oa?id=303026475008 |