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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual de Maringá
2014
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| Online Access: | https://www.redalyc.org/articulo.oa?id=303029495010 |
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Table of Contents:
- Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 Giovana Bomfim de Alcantara Roberson Dibax Ricardo Augusto de Oliveira João Carlos Bespalhok Filho Edelclaiton Daros Agrociencias anatomy Poaceae Tissue culture somatic embryos The development of an efficient regeneration system is crucial for the genetic transformation of sugarcane. The aims of this study were to regenerate plants from somatic embryos and analyze the origin and division pattern of cells during the different embryonic developmental stages of the sugarcane cultivars RB855156 and RB72454. For both cultivars, the best results for embryogenic callus induction were obtained with explant cultures on culture medium containing 13.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by callus culture on solid medium with 4.5 μM 2,4-D. A higher rate of shoot induction was observed in RB855156 with the addition of 8.9 and 17.8 μM benzylaminopurine (BAP); this higher rate was also observed in RB72454 with the addition of 17.8 μM BAP. Murashige and Skoog (1962) (MS) medium containing 2.5 and 5.0 μM indolebutyric acid (IBA) for RB855156 and RB72454, respectively, was suitable for rooting. The plantlets were successfully acclimatized and the plant survival rate was 100% for both cultivars. Histological analysis revealed that the shoots in both cultivars originated from somatic embryogenesis. 2014 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303029495010 en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Num.1 Vol.36