AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidade Estadual de Maringá
2016
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| _version_ | 1876486928919953408 |
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| author | Felipe Aranha de Andrade |
| author_facet | Felipe Aranha de Andrade |
| contents | AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions Felipe Aranha de Andrade Leandro Simões Azeredo Gonçalves Édison Miglioranza Claudete de Fátima Ruas Paulo Mauricio Ruas Lúcia Sadayo Assari Takahashi Agrociencias gene bank molecular markers Phaseolus vulgaris L amplified fragment length polymorphism The present study aimed to estimate and characterize the genetic divergence between determinateand indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bankbased on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions withdeterminate and indeterminate growth habits, respectively, were characterized for this purpose. Sevencombinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and thecombinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. These selective AFLPcombinations revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatestnumber of informative loci (49%). The analysis of dissimilarity frequency distribution showed that thedistribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions withindeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. Theclustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is apossible association between snap bean growth habit and gene pool. The determinate accessions may be moreclosely associated with the Andean gene pool, while the indeterminate ones may be associated with theMesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggestingintrogression between the Andean and Mesoamerican gene pools. 2016 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303044042004 https://www.redalyc.org/journal/3030/303044042004/ https://www.redalyc.org/journal/3030/303044042004/html/ https://www.redalyc.org/journal/3030/303044042004/303044042004.epub https://www.redalyc.org/journal/3030/303044042004/movil en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Num.1 Vol.38 |
| format | Artículo científico |
| id | redalyc_303044042004 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions Felipe Aranha de Andrade Agrociencias gene bank molecular markers Phaseolus vulgaris L amplified fragment length polymorphism AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions Felipe Aranha de Andrade Leandro Simões Azeredo Gonçalves Édison Miglioranza Claudete de Fátima Ruas Paulo Mauricio Ruas Lúcia Sadayo Assari Takahashi Agrociencias gene bank molecular markers Phaseolus vulgaris L amplified fragment length polymorphism The present study aimed to estimate and characterize the genetic divergence between determinateand indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bankbased on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions withdeterminate and indeterminate growth habits, respectively, were characterized for this purpose. Sevencombinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and thecombinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. These selective AFLPcombinations revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected the greatestnumber of informative loci (49%). The analysis of dissimilarity frequency distribution showed that thedistribution was uniform, ranging from 0.1285 to 0.7310 with a mean of 0.4801, and the accessions withindeterminate growth habits exhibited greater variability than the accessions with determinate growth habits. Theclustering UPGMA, PCoA and Bayesian analyses showed the formation of two large clusters, wherein there is apossible association between snap bean growth habit and gene pool. The determinate accessions may be moreclosely associated with the Andean gene pool, while the indeterminate ones may be associated with theMesoamerican gene pool. The Bayesian analysis showed accessions intermediate to both groups, suggestingintrogression between the Andean and Mesoamerican gene pools. 2016 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303044042004 https://www.redalyc.org/journal/3030/303044042004/ https://www.redalyc.org/journal/3030/303044042004/html/ https://www.redalyc.org/journal/3030/303044042004/303044042004.epub https://www.redalyc.org/journal/3030/303044042004/movil en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Num.1 Vol.38 |
| title | AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions |
| topic | Agrociencias gene bank molecular markers Phaseolus vulgaris L amplified fragment length polymorphism |
| url | https://www.redalyc.org/articulo.oa?id=303044042004 https://www.redalyc.org/journal/3030/303044042004/ https://www.redalyc.org/journal/3030/303044042004/html/ https://www.redalyc.org/journal/3030/303044042004/303044042004.epub https://www.redalyc.org/journal/3030/303044042004/movil |