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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual de Maringá
2021
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| Online Access: | https://www.redalyc.org/articulo.oa?id=303067924005 https://www.redalyc.org/journal/3030/303067924005/ https://www.redalyc.org/journal/3030/303067924005/html/ https://www.redalyc.org/journal/3030/303067924005/303067924005.epub https://www.redalyc.org/journal/3030/303067924005/movil |
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| _version_ | 1866814761077833728 |
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| author | Theodoro Schneider |
| author_facet | Theodoro Schneider |
| contents | Expression of genes related to the mechanism of resistance of Conyza sumatrensis to glyphosate Theodoro Schneider Mauro Antônio Rizzardi Magali Ferrari Grando Cássia Canzi Ceccon Anderson Luis Nunes Dielli Didone Agrociencias EPSPs overexpression Sumatran fleabane ABC transport protein genes The elucidation of the resistance mechanism of weeds to herbicides is important for management practices. The objective of this work was to investigate the resistance mechanism of glyphosate-resistant C. sumatrensis biotypes by determining the expression levels of the constitutive gene epsps and two ABC transport protein-coding genes designated m7 and m11 with RT-qPCR. Two biotypes of C. sumatrensis were evaluated: one resistant and one susceptible to glyphosate. The treatments consisted of the absence or application of two doses of glyphosate (1,080 and 8,640 g a.e. ha-1). Plant leaves were collected at 1 and 4 days after herbicide application. No difference was observed in epsps gene expression between the studied biotypes. The expression of the m7 and m11 genes revealed that both genes had higher relative expression in the resistant biotype with the application of glyphosate at both doses. The overexpression of the m7 and m11 genes in the resistant biotype treated with glyphosate reveals that these genes play a role in herbicide resistance. These genes may be involved in the sequestration of glyphosate into the vacuole lumen in the resistant C. sumatrensis biotype studied. 2021 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303067924005 https://www.redalyc.org/journal/3030/303067924005/ https://www.redalyc.org/journal/3030/303067924005/html/ https://www.redalyc.org/journal/3030/303067924005/303067924005.epub https://www.redalyc.org/journal/3030/303067924005/movil 10.4025/actasciagron.v43i1.44824 en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Vol.43 |
| format | Artículo científico |
| id | redalyc_303067924005 |
| language | en |
| publishDate | 2021 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Expression of genes related to the mechanism of resistance of Conyza sumatrensis to glyphosate Theodoro Schneider Agrociencias EPSPs overexpression Sumatran fleabane ABC transport protein genes Expression of genes related to the mechanism of resistance of Conyza sumatrensis to glyphosate Theodoro Schneider Mauro Antônio Rizzardi Magali Ferrari Grando Cássia Canzi Ceccon Anderson Luis Nunes Dielli Didone Agrociencias EPSPs overexpression Sumatran fleabane ABC transport protein genes The elucidation of the resistance mechanism of weeds to herbicides is important for management practices. The objective of this work was to investigate the resistance mechanism of glyphosate-resistant C. sumatrensis biotypes by determining the expression levels of the constitutive gene epsps and two ABC transport protein-coding genes designated m7 and m11 with RT-qPCR. Two biotypes of C. sumatrensis were evaluated: one resistant and one susceptible to glyphosate. The treatments consisted of the absence or application of two doses of glyphosate (1,080 and 8,640 g a.e. ha-1). Plant leaves were collected at 1 and 4 days after herbicide application. No difference was observed in epsps gene expression between the studied biotypes. The expression of the m7 and m11 genes revealed that both genes had higher relative expression in the resistant biotype with the application of glyphosate at both doses. The overexpression of the m7 and m11 genes in the resistant biotype treated with glyphosate reveals that these genes play a role in herbicide resistance. These genes may be involved in the sequestration of glyphosate into the vacuole lumen in the resistant C. sumatrensis biotype studied. 2021 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303067924005 https://www.redalyc.org/journal/3030/303067924005/ https://www.redalyc.org/journal/3030/303067924005/html/ https://www.redalyc.org/journal/3030/303067924005/303067924005.epub https://www.redalyc.org/journal/3030/303067924005/movil 10.4025/actasciagron.v43i1.44824 en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Vol.43 |
| title | Expression of genes related to the mechanism of resistance of Conyza sumatrensis to glyphosate |
| topic | Agrociencias EPSPs overexpression Sumatran fleabane ABC transport protein genes |
| url | https://www.redalyc.org/articulo.oa?id=303067924005 https://www.redalyc.org/journal/3030/303067924005/ https://www.redalyc.org/journal/3030/303067924005/html/ https://www.redalyc.org/journal/3030/303067924005/303067924005.epub https://www.redalyc.org/journal/3030/303067924005/movil |