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Main Author:	Bárbara Nogueira Souza Costa
Format:	Artículo científico
Language:	en
Published:	Universidade Estadual de Maringá 2021
Subjects:	Agrociencias Musa spp bioreactor culture media potassium silicate
Online Access:	https://www.redalyc.org/articulo.oa?id=303067924019 https://www.redalyc.org/journal/3030/303067924019/ https://www.redalyc.org/journal/3030/303067924019/html/ https://www.redalyc.org/journal/3030/303067924019/303067924019.epub https://www.redalyc.org/journal/3030/303067924019/movil
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Influence of silicon and in vitro culture systems on the micropropagation and acclimatization of “Dwarf Cavendish” banana Bárbara Nogueira Souza Costa Aurélio Rúbio Edvan Alves Chagas Pollyana Cardoso Chagas Moacir Pasqual Wagner Aparecido Vendrame Agrociencias Musa spp bioreactor culture media potassium silicate In vitro culture systems based on liquid culture media are considered to be more effective than semisolid culture medium systems. Liquid culture media systems provide better nutrient availability for plant tissues, easier culture handling, and the potential for scaling up and automation. However, in vitro liquid culture requires more careful handling due to the potential for contamination and the possibility of negative effects, such as hyperhydricity or vitrification, that hinder the growth and development of the plant material. Temporary immersion bioreactors have emerged as a workable alternative for capturing the benefits of liquid media, though semisolid systems are still traditional. Many studies have shown that silicon (Si) is a beneficial plant nutrient. Silicon might have a positive effect in both semisolid and liquid in vitro systems. The objective of this study was to evaluate the effect of silicon on the micropropagation and acclimatization of banana plants cultivated in vitro by comparing liquid temporary immersion bioreactor technology and semisolid traditional culture systems. Different silicon concentrations (0 and 1 mL L-1) and culture systems (liquid temporary immersion bioreactor and semisolid traditional culture) were evaluated over a 36-day period. The growth characteristics plant size, fresh and dry weight, and number and length of leaves and roots were evaluated. After the 36-day in vitro growth period, plants were transferred to a greenhouse for acclimatization and were evaluated after 30 days for the same growth characteristics used in the in vitro studies. The temporary immersion bioreactor system resulted in greater growth of banana plants compared to the traditional semisolid system. Temporary immersion bioreactors also showed a positive interaction with Si and resulted in higher values for all growth characteristics in the acclimatization phase. 2021 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303067924019 https://www.redalyc.org/journal/3030/303067924019/ https://www.redalyc.org/journal/3030/303067924019/html/ https://www.redalyc.org/journal/3030/303067924019/303067924019.epub https://www.redalyc.org/journal/3030/303067924019/movil 10.4025/actasciagron.v43i1.47490 en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Vol.43

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