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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual de Maringá
2023
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| Online Access: | https://www.redalyc.org/articulo.oa?id=303075473003 https://www.redalyc.org/journal/3030/303075473003/ https://www.redalyc.org/journal/3030/303075473003/html/ https://www.redalyc.org/journal/3030/303075473003/303075473003.epub https://www.redalyc.org/journal/3030/303075473003/movil |
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Table of Contents:
- A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCR Suzana Marjorie Freire e Silva Gilsivan Sales Medeiros de Aquino Tálison Eugênio da Costa Anna Luisa de Carvalho Brito Andréia Mitsa Paiva Negreiros Rui Sales Tatsuya Nagata Ioná Araújo Santos Holanda Agrociencias direct PCR ITS primer Charcoal rot Citrullus lanatus Macrophomina phaseolina (Tassi) Goid is the causal agent of charcoal rot and vine decline in cucurbits such as watermelon. Molecular methods have been used for rapid identification. However, a large number of steps used reduces its applicability. This study aimed to detect M. phaseolina in watermelon from producing areas in Northeastern Brazil by direct PCR. Plant tissue samples were collected from seven producing areas and the DNA was extracted using the CTAB method. Amplifications were performed by direct PCR using the MpKFI/MpKRI primers, then the PCR products were subjected to agarose gel electrophoresis and sequenced. Amplicons of 350 bp were observed in stem tissue samples from three areas. The identity of the samples was confirmed by sequencing. This study represents the first molecular diagnosis of M. phaseolina associated with watermelon in Northeastern Brazil. The methodology presented here can be applied for a reliable and simple diagnosis of the pathogen in other crops. 2023 artículo científico 1679-9275 https://www.redalyc.org/articulo.oa?id=303075473003 https://www.redalyc.org/journal/3030/303075473003/ https://www.redalyc.org/journal/3030/303075473003/html/ https://www.redalyc.org/journal/3030/303075473003/303075473003.epub https://www.redalyc.org/journal/3030/303075473003/movil 10.4025/actasciagron.v45i1.56141 en http://www.redalyc.org/revista.oa?id=3030 Acta Scientiarum. Agronomy application/pdf Universidade Estadual de Maringá Acta Scientiarum. Agronomy (Brasil) Vol.45