Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidade Estadual de Maringá
2015
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| _version_ | 1876458258210750464 |
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| author | Raphael Barbetta de Jesus |
| author_facet | Raphael Barbetta de Jesus |
| contents | Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy Raphael Barbetta de Jesus Wellington Pine Omori Eliana Gertrudes de Macedo Lemos Jackson Antônio Marcondes de Souza Agrociencias Nellore Nellore Prevotella Prevotella bacterioidetes The bacterial diversity by 16S rDNA partial sequencing and scanning electron microscope (SEM) of the rumen microbiome was characterized. Three Nellore bovines, cannulated at the rumen, were utilized. Liquid and solid fractions from the rumen content were processed for the extraction of metagenomic DNA and later 16S rDNA amplicons were utilized to construct the WGA library for further clone sequencing. Data were analyzed by MEGA and MOTUR (University of Michigan) softwares. Approximately 97.96% of operation taxonomic units (OTUs) were related to Bacteriodetes phylum and 2.04% of sequences were affiliated to Firmicutes phylum. In the case of Bacteriodetes, the great part of sequences (47.96%) was attributed to Prevotella genus. Bacteroidetes phylum was predominant in rumen content and the Prevotella genus was the most abundant, including di verse species related to this taxon. The bacterial morphological diversity associated to plant fibers was detected by SEM and showed its role in plant biomass deconstruction beyond the detection of microbiological interactions that involved protozoa. 2015 artículo científico 1806-2636 https://www.redalyc.org/articulo.oa?id=303141017006 en http://www.redalyc.org/revista.oa?id=3031 Acta Scientiarum. Animal Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Animal Sciences (Brasil) Num.3 Vol.37 |
| format | Artículo científico |
| id | redalyc_303141017006 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy Raphael Barbetta de Jesus Agrociencias Nellore Nellore Prevotella Prevotella bacterioidetes Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy Raphael Barbetta de Jesus Wellington Pine Omori Eliana Gertrudes de Macedo Lemos Jackson Antônio Marcondes de Souza Agrociencias Nellore Nellore Prevotella Prevotella bacterioidetes The bacterial diversity by 16S rDNA partial sequencing and scanning electron microscope (SEM) of the rumen microbiome was characterized. Three Nellore bovines, cannulated at the rumen, were utilized. Liquid and solid fractions from the rumen content were processed for the extraction of metagenomic DNA and later 16S rDNA amplicons were utilized to construct the WGA library for further clone sequencing. Data were analyzed by MEGA and MOTUR (University of Michigan) softwares. Approximately 97.96% of operation taxonomic units (OTUs) were related to Bacteriodetes phylum and 2.04% of sequences were affiliated to Firmicutes phylum. In the case of Bacteriodetes, the great part of sequences (47.96%) was attributed to Prevotella genus. Bacteroidetes phylum was predominant in rumen content and the Prevotella genus was the most abundant, including di verse species related to this taxon. The bacterial morphological diversity associated to plant fibers was detected by SEM and showed its role in plant biomass deconstruction beyond the detection of microbiological interactions that involved protozoa. 2015 artículo científico 1806-2636 https://www.redalyc.org/articulo.oa?id=303141017006 en http://www.redalyc.org/revista.oa?id=3031 Acta Scientiarum. Animal Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Animal Sciences (Brasil) Num.3 Vol.37 |
| title | Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy |
| topic | Agrociencias Nellore Nellore Prevotella Prevotella bacterioidetes |
| url | https://www.redalyc.org/articulo.oa?id=303141017006 |