L-asparaginase production by Zymomonas mobilis during molasses fermentation: optimization of culture conditions using factorial design
Fuente:
Redalyc
Salvato in:
| Autore principale: | |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Universidade Estadual de Maringá
2006
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1876423670301196288 |
|---|---|
| author | Doumit Camilios Neto |
| author_facet | Doumit Camilios Neto |
| contents | L-asparaginase production by Zymomonas mobilis during molasses fermentation: optimization of culture conditions using factorial design Doumit Camilios Neto João Batista Buzato Dionísio Borsato Ingeniería asparaginase factorial design Zymomonas mobilis sugarcane molasses L-asparaginase is an enzyme with anti-leukemia activity produced by microorganisms especially gram-negative bacteria. Zymomonas mobilis is a gram-negative bacteria that presents potential for L-asparaginase production. This study sought to optimize L-asparaginase production by Zymomonas mobilis, during sugarcane molasses fermentation, using an incomplete 33 factorial design. A model obtained by the response surface methodology was optimized by Otplex software. Good fit of the experimental data to the model was obtained, and the total determination coefficient (R2) was 95%. Maximal enzyme activity (16.55 IU L-1) was detected under the following conditions, of molasses concentration 100.0 g L-1 of total reducing sugars, yeast extract concentration 2.0 g L-1 and fermentation time 21 hours. The experimental validation confirmed the predictive capacity of the model, and the difference between the estimated response (Ŷ 1) and the observed response (Y1) was only 1%. 2006 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303226516002 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Num.2 Vol.28 |
| format | Artículo científico |
| id | redalyc_303226516002 |
| institution | Redalyc |
| language | en |
| publishDate | 2006 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | L-asparaginase production by Zymomonas mobilis during molasses fermentation: optimization of culture conditions using factorial design Doumit Camilios Neto Ingeniería asparaginase factorial design Zymomonas mobilis sugarcane molasses L-asparaginase production by Zymomonas mobilis during molasses fermentation: optimization of culture conditions using factorial design Doumit Camilios Neto João Batista Buzato Dionísio Borsato Ingeniería asparaginase factorial design Zymomonas mobilis sugarcane molasses L-asparaginase is an enzyme with anti-leukemia activity produced by microorganisms especially gram-negative bacteria. Zymomonas mobilis is a gram-negative bacteria that presents potential for L-asparaginase production. This study sought to optimize L-asparaginase production by Zymomonas mobilis, during sugarcane molasses fermentation, using an incomplete 33 factorial design. A model obtained by the response surface methodology was optimized by Otplex software. Good fit of the experimental data to the model was obtained, and the total determination coefficient (R2) was 95%. Maximal enzyme activity (16.55 IU L-1) was detected under the following conditions, of molasses concentration 100.0 g L-1 of total reducing sugars, yeast extract concentration 2.0 g L-1 and fermentation time 21 hours. The experimental validation confirmed the predictive capacity of the model, and the difference between the estimated response (Ŷ 1) and the observed response (Y1) was only 1%. 2006 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303226516002 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Num.2 Vol.28 |
| title | L-asparaginase production by Zymomonas mobilis during molasses fermentation: optimization of culture conditions using factorial design |
| topic | Ingeniería asparaginase factorial design Zymomonas mobilis sugarcane molasses |
| url | https://www.redalyc.org/articulo.oa?id=303226516002 |