Evaluation of temperature and moisture response surface on the lipase from pumpkin seeds fermentation using Aspergillus niger
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidade Estadual de Maringá
2012
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| _version_ | 1876447172449271808 |
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| author | Rafaela Cristiane Andrade Santos |
| author_facet | Rafaela Cristiane Andrade Santos |
| contents | Evaluation of temperature and moisture response surface on the lipase from pumpkin seeds fermentation using Aspergillus niger Rafaela Cristiane Andrade Santos Kyzzes Barreto Araújo Cleide Mara Faria Soares Luciana Cristina Lins de Aquino Ingeniería Agro solid state fermentation industrial waste microbial enzyme Agro-industrial waste has shown great potential for use in solid-state fermentation due to its low cost and promoting the growth of microorganisms. This study evaluated the interaction between temperature and moisture response surface on lipase from the fermentation of pumpkin seed flour, using Aspergillus niger. Fermentations were performed in Petri dishes in an experimental design using a 22 matrix with four replications at the center point and four axial points, varying substrate moisture between 30 and 60% and temperature between 30 and 40ºC. The maximum hydrolytic activity (71.88 U g-1 dry weight) was obtained using flour with 30% moisture and 30°C temperature in 120 hours of fermentation. The interaction between fermentation temperature and initial moisture content of the residue was the parameter that most influenced the process at 95% significance level. The response surface analysis showed that the maximum lipase production can be obtained under temperature and moisture conditions of the flour ranging from 28 to 34.5°C and 25 to 32.5%; or between 32 and 40°C and 58 and 65%, respectively. Pumpkin seed flour showed potential for obtaining microbial lipase. 2012 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303226542001 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Num.3 Vol.34 |
| format | Artículo científico |
| id | redalyc_303226542001 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Evaluation of temperature and moisture response surface on the lipase from pumpkin seeds fermentation using Aspergillus niger Rafaela Cristiane Andrade Santos Ingeniería Agro solid state fermentation industrial waste microbial enzyme Evaluation of temperature and moisture response surface on the lipase from pumpkin seeds fermentation using Aspergillus niger Rafaela Cristiane Andrade Santos Kyzzes Barreto Araújo Cleide Mara Faria Soares Luciana Cristina Lins de Aquino Ingeniería Agro solid state fermentation industrial waste microbial enzyme Agro-industrial waste has shown great potential for use in solid-state fermentation due to its low cost and promoting the growth of microorganisms. This study evaluated the interaction between temperature and moisture response surface on lipase from the fermentation of pumpkin seed flour, using Aspergillus niger. Fermentations were performed in Petri dishes in an experimental design using a 22 matrix with four replications at the center point and four axial points, varying substrate moisture between 30 and 60% and temperature between 30 and 40ºC. The maximum hydrolytic activity (71.88 U g-1 dry weight) was obtained using flour with 30% moisture and 30°C temperature in 120 hours of fermentation. The interaction between fermentation temperature and initial moisture content of the residue was the parameter that most influenced the process at 95% significance level. The response surface analysis showed that the maximum lipase production can be obtained under temperature and moisture conditions of the flour ranging from 28 to 34.5°C and 25 to 32.5%; or between 32 and 40°C and 58 and 65%, respectively. Pumpkin seed flour showed potential for obtaining microbial lipase. 2012 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303226542001 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Num.3 Vol.34 |
| title | Evaluation of temperature and moisture response surface on the lipase from pumpkin seeds fermentation using Aspergillus niger |
| topic | Ingeniería Agro solid state fermentation industrial waste microbial enzyme |
| url | https://www.redalyc.org/articulo.oa?id=303226542001 |