Use of agro-industrial wastes as substrates for a-amylase production by endophytic fungi isolated from Piper hispidum Sw
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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual de Maringá
2017
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| author | Ravely Casarotti Orlandelli |
| author_facet | Ravely Casarotti Orlandelli |
| contents | Use of agro-industrial wastes as substrates for a-amylase production by endophytic fungi isolated from Piper hispidum Sw Ravely Casarotti Orlandelli Mariana Sanches Santos Julio Cesar Polonio João Lúcio de Azevedo João Alencar Pamphile Ingeniería endophytes microbial enzymes Amylolytic activity submerged fermentation Millions of tons of corn, pineapple, sugarcane and wheat are annually produced and their industrial processing gene rates large amounts of wastes. Current st udy evaluated the use of corncob (CC), pineapple peel (PP), sugarcane bagasse (SB) and wheat bran (WB) as substrates for α -amylase production by submerged cultures of nine endophytes from Piper hispidum Sw. Initially, fungi were grown on a starch medium and the cup plate assay showed that five strains were amylase-positive: Bipolaris sp. JF767001, Marasmius cladophyllus JF767003, Phlebia sp. JF766997, Phyllosticta capitalensis JF766988 and Schizophyllum commune JF766994, with enzymatic halos ranging between 6.30 ± 0.08 and 8.87 ± 0.68 mm in diameter. Further, the use of agro- industrial wastes was evaluated by the cup plate assay, where the highest halo diameters were obtained from fungi grown on PP or SB: up to 15.00 ± 0.16 mm ( Phlebia sp.) and 14.80 ± 0.18 mm ( S. commune ), respectively. Submerged cultures with PP or SB showed the highest levels of α -amylase activity according to the starch-iodine assay, or rather, up to 4.14 ± 0.02 U mL -1 ( Bipolaris sp.) and 4.09 ± 0.02 U mL -1 ( Phlebia sp.), respectively. Results give an in-depth knowledge on tropical endophytes that might be α -amylase sources, and indicate the suitability of these agro-industrial wastes as substrates for fungal enzymatic production. 2017 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303252892001 https://www.redalyc.org/journal/3032/303252892001/ https://www.redalyc.org/journal/3032/303252892001/html/ https://www.redalyc.org/journal/3032/303252892001/303252892001.epub https://www.redalyc.org/journal/3032/303252892001/movil en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Num.3 Vol.39 |
| format | Artículo científico |
| id | redalyc_303252892001 |
| institution | Redalyc |
| language | en |
| publishDate | 2017 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Use of agro-industrial wastes as substrates for a-amylase production by endophytic fungi isolated from Piper hispidum Sw Ravely Casarotti Orlandelli Ingeniería endophytes microbial enzymes Amylolytic activity submerged fermentation Use of agro-industrial wastes as substrates for a-amylase production by endophytic fungi isolated from Piper hispidum Sw Ravely Casarotti Orlandelli Mariana Sanches Santos Julio Cesar Polonio João Lúcio de Azevedo João Alencar Pamphile Ingeniería endophytes microbial enzymes Amylolytic activity submerged fermentation Millions of tons of corn, pineapple, sugarcane and wheat are annually produced and their industrial processing gene rates large amounts of wastes. Current st udy evaluated the use of corncob (CC), pineapple peel (PP), sugarcane bagasse (SB) and wheat bran (WB) as substrates for α -amylase production by submerged cultures of nine endophytes from Piper hispidum Sw. Initially, fungi were grown on a starch medium and the cup plate assay showed that five strains were amylase-positive: Bipolaris sp. JF767001, Marasmius cladophyllus JF767003, Phlebia sp. JF766997, Phyllosticta capitalensis JF766988 and Schizophyllum commune JF766994, with enzymatic halos ranging between 6.30 ± 0.08 and 8.87 ± 0.68 mm in diameter. Further, the use of agro- industrial wastes was evaluated by the cup plate assay, where the highest halo diameters were obtained from fungi grown on PP or SB: up to 15.00 ± 0.16 mm ( Phlebia sp.) and 14.80 ± 0.18 mm ( S. commune ), respectively. Submerged cultures with PP or SB showed the highest levels of α -amylase activity according to the starch-iodine assay, or rather, up to 4.14 ± 0.02 U mL -1 ( Bipolaris sp.) and 4.09 ± 0.02 U mL -1 ( Phlebia sp.), respectively. Results give an in-depth knowledge on tropical endophytes that might be α -amylase sources, and indicate the suitability of these agro-industrial wastes as substrates for fungal enzymatic production. 2017 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303252892001 https://www.redalyc.org/journal/3032/303252892001/ https://www.redalyc.org/journal/3032/303252892001/html/ https://www.redalyc.org/journal/3032/303252892001/303252892001.epub https://www.redalyc.org/journal/3032/303252892001/movil en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Num.3 Vol.39 |
| title | Use of agro-industrial wastes as substrates for a-amylase production by endophytic fungi isolated from Piper hispidum Sw |
| topic | Ingeniería endophytes microbial enzymes Amylolytic activity submerged fermentation |
| url | https://www.redalyc.org/articulo.oa?id=303252892001 https://www.redalyc.org/journal/3032/303252892001/ https://www.redalyc.org/journal/3032/303252892001/html/ https://www.redalyc.org/journal/3032/303252892001/303252892001.epub https://www.redalyc.org/journal/3032/303252892001/movil |