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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidade Estadual de Maringá
2019
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| Online Access: | https://www.redalyc.org/articulo.oa?id=303260200052 https://www.redalyc.org/journal/3032/303260200052/ https://www.redalyc.org/journal/3032/303260200052/html/ https://www.redalyc.org/journal/3032/303260200052/303260200052.epub https://www.redalyc.org/journal/3032/303260200052/movil https://doi.org/10.4025/actascitechnol.v41i1.39693 |
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| _version_ | 1866814764257116160 |
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| author | Maiara Priscilla de Souza |
| author_facet | Maiara Priscilla de Souza |
| contents | Screening of fungal strains with potentiality to hydrolyze microalgal biomass by Fourier Transform Infrared Spectroscopy (FTIR) Maiara Priscilla de Souza Michele Hoeltz Maria Viviane Gomes Muller Pablo Diego Gressler Thiago Rodrigues Bjerk Rosana de Cássia de Souza Schneider Valeriano Antonio Corbellini Ingeniería fungi Chlorella sp infrared spectroscopy Desmodesmus subspicatus The use of fungi is a promising alternative for harnessing biomass after lipid extraction of microalgae since this biomass may contain relevant levels of carbohydrates that can be converted into other compounds of interest, such as sugars. Fourier Transform Infrared Spectroscopy (FTIR) has proved to be an efficient and environmentally less impacting tool for the selection of microorganisms with biotechnological potential. This study aimed to apply FTIR for the selection of fungal strains with potential to hydrolyze the biomass of the microalgae Desmodesmus subspicatus and Chlorella sp after lipid extraction. Eleven fungal strains were screened for residual biomass hydrolysis and FTIR was applied followed by multivariate analysis for the selection of filamentous fungi. The highest cell density was 28.7 × 106 cells mL-1 for Chlorella sp. and 15.8 × 106 cells mL-1 for D. subspicatus and the values of total carbohydrates content were 23.1 and 16.9%, respectively. Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were useful tools to screen fungal strains. After multivariate analysis, it was possible to observe that the fungi strains that presented the greatest ability to use microalgal biomass were Penicillium G12 due to the glucose and xylose sugars obtained after lipid extraction from D. subspicatus (with sugar yield of 9.4 and 6.6%, respectively) and Trichoderma auricularis for Chlorella sp. (with sugar yield of 12.9 and 9.6%, respectively). FTIR was successfully applied to screen fungal strains. 2019 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303260200052 https://www.redalyc.org/journal/3032/303260200052/ https://www.redalyc.org/journal/3032/303260200052/html/ https://www.redalyc.org/journal/3032/303260200052/303260200052.epub https://www.redalyc.org/journal/3032/303260200052/movil https://doi.org/10.4025/actascitechnol.v41i1.39693 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Vol.41 |
| format | Artículo científico |
| id | redalyc_303260200052 |
| language | en |
| publishDate | 2019 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Screening of fungal strains with potentiality to hydrolyze microalgal biomass by Fourier Transform Infrared Spectroscopy (FTIR) Maiara Priscilla de Souza Ingeniería fungi Chlorella sp infrared spectroscopy Desmodesmus subspicatus Screening of fungal strains with potentiality to hydrolyze microalgal biomass by Fourier Transform Infrared Spectroscopy (FTIR) Maiara Priscilla de Souza Michele Hoeltz Maria Viviane Gomes Muller Pablo Diego Gressler Thiago Rodrigues Bjerk Rosana de Cássia de Souza Schneider Valeriano Antonio Corbellini Ingeniería fungi Chlorella sp infrared spectroscopy Desmodesmus subspicatus The use of fungi is a promising alternative for harnessing biomass after lipid extraction of microalgae since this biomass may contain relevant levels of carbohydrates that can be converted into other compounds of interest, such as sugars. Fourier Transform Infrared Spectroscopy (FTIR) has proved to be an efficient and environmentally less impacting tool for the selection of microorganisms with biotechnological potential. This study aimed to apply FTIR for the selection of fungal strains with potential to hydrolyze the biomass of the microalgae Desmodesmus subspicatus and Chlorella sp after lipid extraction. Eleven fungal strains were screened for residual biomass hydrolysis and FTIR was applied followed by multivariate analysis for the selection of filamentous fungi. The highest cell density was 28.7 × 106 cells mL-1 for Chlorella sp. and 15.8 × 106 cells mL-1 for D. subspicatus and the values of total carbohydrates content were 23.1 and 16.9%, respectively. Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were useful tools to screen fungal strains. After multivariate analysis, it was possible to observe that the fungi strains that presented the greatest ability to use microalgal biomass were Penicillium G12 due to the glucose and xylose sugars obtained after lipid extraction from D. subspicatus (with sugar yield of 9.4 and 6.6%, respectively) and Trichoderma auricularis for Chlorella sp. (with sugar yield of 12.9 and 9.6%, respectively). FTIR was successfully applied to screen fungal strains. 2019 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303260200052 https://www.redalyc.org/journal/3032/303260200052/ https://www.redalyc.org/journal/3032/303260200052/html/ https://www.redalyc.org/journal/3032/303260200052/303260200052.epub https://www.redalyc.org/journal/3032/303260200052/movil https://doi.org/10.4025/actascitechnol.v41i1.39693 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Vol.41 |
| title | Screening of fungal strains with potentiality to hydrolyze microalgal biomass by Fourier Transform Infrared Spectroscopy (FTIR) |
| topic | Ingeniería fungi Chlorella sp infrared spectroscopy Desmodesmus subspicatus |
| url | https://www.redalyc.org/articulo.oa?id=303260200052 https://www.redalyc.org/journal/3032/303260200052/ https://www.redalyc.org/journal/3032/303260200052/html/ https://www.redalyc.org/journal/3032/303260200052/303260200052.epub https://www.redalyc.org/journal/3032/303260200052/movil https://doi.org/10.4025/actascitechnol.v41i1.39693 |