Influence of optimised commercial medium on bacteriocin production by Enterococcus faecium
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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual de Maringá
2019
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| _version_ | 1876437627714928640 |
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| author | Luciana Furlaneto Maia |
| author_facet | Luciana Furlaneto Maia |
| contents | Influence of optimised commercial medium on bacteriocin production by Enterococcus faecium Luciana Furlaneto Maia Larissa Cristina Costa Kátia Real Rocha Márcia Regina Terra Janaína Schueller Natara Favaro Tosoni Márcia Cristina Furlaneto Ingeniería inhibition Bioconservants Listeria innocua chemical products Enterocins carry out antimicrobial activity on several pathogenic and spoilage bacteria in food. The objective of this work was to evaluate the production of enterocins, using Enterococcus faecium, and their stability under different culture conditions. The isolate was incubated in ManRogosa Sharpe (MRS) medium with pHs 4.5, 5.0, 5.5, 6.0 and 6.5, and supplemented with lactose, mannose, mannitol, glycerol, tween 20, and tween 80. The optimum pH of production was between 6.0 and 6.5. The enterocin produced in mix (lactose 20, glycerol 20, tween20 5.0 g L-1) was more efficient. None pH tested interfered with the reduction of the action of the enterocins. The thermal stability varied according to the isolate as well as the supplementation used. Tween 20 and 80 with regards to the stability to different chemical products of both isolates, presented reduction of the action. It was observed that the supplement mix had higher adsorption and that the bactericidal effect was observed within 2 hours of incubation. The results indicated that the different culture conditions did not affected the antagonistic action of enterocin. 2019 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303260200060 https://www.redalyc.org/journal/3032/303260200060/ https://www.redalyc.org/journal/3032/303260200060/html/ https://www.redalyc.org/journal/3032/303260200060/303260200060.epub https://www.redalyc.org/journal/3032/303260200060/movil https://doi.org/10.4025/actascitechnol.v41i1.42324 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Vol.41 |
| format | Artículo científico |
| id | redalyc_303260200060 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Influence of optimised commercial medium on bacteriocin production by Enterococcus faecium Luciana Furlaneto Maia Ingeniería inhibition Bioconservants Listeria innocua chemical products Influence of optimised commercial medium on bacteriocin production by Enterococcus faecium Luciana Furlaneto Maia Larissa Cristina Costa Kátia Real Rocha Márcia Regina Terra Janaína Schueller Natara Favaro Tosoni Márcia Cristina Furlaneto Ingeniería inhibition Bioconservants Listeria innocua chemical products Enterocins carry out antimicrobial activity on several pathogenic and spoilage bacteria in food. The objective of this work was to evaluate the production of enterocins, using Enterococcus faecium, and their stability under different culture conditions. The isolate was incubated in ManRogosa Sharpe (MRS) medium with pHs 4.5, 5.0, 5.5, 6.0 and 6.5, and supplemented with lactose, mannose, mannitol, glycerol, tween 20, and tween 80. The optimum pH of production was between 6.0 and 6.5. The enterocin produced in mix (lactose 20, glycerol 20, tween20 5.0 g L-1) was more efficient. None pH tested interfered with the reduction of the action of the enterocins. The thermal stability varied according to the isolate as well as the supplementation used. Tween 20 and 80 with regards to the stability to different chemical products of both isolates, presented reduction of the action. It was observed that the supplement mix had higher adsorption and that the bactericidal effect was observed within 2 hours of incubation. The results indicated that the different culture conditions did not affected the antagonistic action of enterocin. 2019 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303260200060 https://www.redalyc.org/journal/3032/303260200060/ https://www.redalyc.org/journal/3032/303260200060/html/ https://www.redalyc.org/journal/3032/303260200060/303260200060.epub https://www.redalyc.org/journal/3032/303260200060/movil https://doi.org/10.4025/actascitechnol.v41i1.42324 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Vol.41 |
| title | Influence of optimised commercial medium on bacteriocin production by Enterococcus faecium |
| topic | Ingeniería inhibition Bioconservants Listeria innocua chemical products |
| url | https://www.redalyc.org/articulo.oa?id=303260200060 https://www.redalyc.org/journal/3032/303260200060/ https://www.redalyc.org/journal/3032/303260200060/html/ https://www.redalyc.org/journal/3032/303260200060/303260200060.epub https://www.redalyc.org/journal/3032/303260200060/movil https://doi.org/10.4025/actascitechnol.v41i1.42324 |