Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidade Estadual de Maringá
2021
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=303271763021 https://www.redalyc.org/journal/3032/303271763021/ https://www.redalyc.org/journal/3032/303271763021/html/ https://www.redalyc.org/journal/3032/303271763021/303271763021.epub https://www.redalyc.org/journal/3032/303271763021/movil https://doi.org/10.4025/actascitechnol.v43i1.49826 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- Bioactive compounds in the leaves of Baccharis dracunculifolia: extraction process and characterization Genice Iurckevicz Débora Dahmer Maira Casagrande Gabrielle Cristina Calegari Tatiane Luiza Cadorin Oldoni Robert Frans Huibert Dekker Carlos Ricardo Maneck Malfatti Mário Antônio Alves Cunha Ingeniería leaf extracts natural products Biological activity experimental design Baccharis dracunculifolia has been used in popular medicine because of its bioactive properties, which include anti-inflammatory, antiulcerative and hepatoprotective potentials. A 2³ central composite rotational design was used to define the best conditions for extracting the bioactive compounds from the leaves of Baccharis dracunculifolia. The parameters studied included the solvent concentration, time and temperature of extraction and the influence of these on the total phenolics content and antioxidant activity. The best conditions were 95% methanol (v/v), 20 min extraction time, and 70°C temperature. Under the optimized conditions, high contents of total phenolic compounds (72.5 mg GAE g-1) and high DPPH (865.6 µmol TE g-1) and ABTS (331.11 μmol TE g-1) free radical scavenging capacity and a high ferric reducing antioxidant power (1558.00 μmol FeSO..7H.O g-1), were obtained. The extract also showed promising anti-microbial activity, demonstrating fungistatic action against C. tropicalis and C. albicans (5 mg mL-1 and 7 mg mL-1, respectively) and bacteriostatic effect against Escherichia coli (60 e 70 mg mL-1), Salmonella enterica typhimurium (40; 60 and 70 mg mL-1) and Staphylococcus aureus (4 mg mL-1). HPLC-DAD analysis showed an extract rich in the phenolic compounds catechin (0.659 mg g-1), caffeic acid (1.135 mg g-1), .-coumaric acid (0.772 mg g-1), ferulic acid (6.24 mg g-1), pinocembrina (0.155 mg g-1) and rutin (0.037 mg g-1). 2021 artículo científico 1806-2563 https://www.redalyc.org/articulo.oa?id=303271763021 https://www.redalyc.org/journal/3032/303271763021/ https://www.redalyc.org/journal/3032/303271763021/html/ https://www.redalyc.org/journal/3032/303271763021/303271763021.epub https://www.redalyc.org/journal/3032/303271763021/movil https://doi.org/10.4025/actascitechnol.v43i1.49826 en http://www.redalyc.org/revista.oa?id=3032 Acta Scientiarum. Technology application/pdf Universidade Estadual de Maringá Acta Scientiarum. Technology (Brasil) Vol.43