Genetic diversity of Trypanosoma cruzi natural populations in Paraná state, southern Brazil
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidade Estadual de Maringá
2007
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| _version_ | 1876491316838268928 |
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| author | Leila Zalloum |
| author_facet | Leila Zalloum |
| contents | Genetic diversity of Trypanosoma cruzi natural populations in Paraná state, southern Brazil Leila Zalloum Mônica Lúcia Gomes Andréia Tieko Kinoshita Max Jean de Ornelas Toledo Alberto José Prioli Silvana Marques Araújo Medicina SSR PCR RAPD genetic groups Trypanosoma cruzi The genetic characteristics of twelve Trypanosoma cruzi strains isolated from chronic chagasic patients, triatomines and sylvatic reservoirs from Paraná state, Southern Brazil, were studied using the randomly amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored polymerase chain reaction amplification (SSR-PCR) techniques. Sylvio and Esmeraldo stocks were used as reference for T. cruzi I and II, respectively. The DNA was amplified using three different primers for RAPD and (CA)8RY primer for SSRPCR. Gel analyses were scored by eye for a numerical taxonomy analysis based on the proportion of shared bands and by employing computer programs. The phenograms showed the existence of two distinct genetic groups: one containing the strains from chronic chagasic patients grouped with Esmeraldo and the other with the strains from triatomines and sylvatic reservoirs grouped with Sylvio. The existence of two phylogenetic groups of T. cruzi in Paraná state, Southern Brazil, is described for the first time. 2007 artículo científico 1679-9291 https://www.redalyc.org/articulo.oa?id=307226620004 en http://www.redalyc.org/revista.oa?id=3072 Acta Scientiarum. Health Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Health Sciences (Brasil) Num.1 Vol.29 |
| format | Artículo científico |
| id | redalyc_307226620004 |
| institution | Redalyc |
| language | en |
| publishDate | 2007 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Genetic diversity of Trypanosoma cruzi natural populations in Paraná state, southern Brazil Leila Zalloum Medicina SSR PCR RAPD genetic groups Trypanosoma cruzi Genetic diversity of Trypanosoma cruzi natural populations in Paraná state, southern Brazil Leila Zalloum Mônica Lúcia Gomes Andréia Tieko Kinoshita Max Jean de Ornelas Toledo Alberto José Prioli Silvana Marques Araújo Medicina SSR PCR RAPD genetic groups Trypanosoma cruzi The genetic characteristics of twelve Trypanosoma cruzi strains isolated from chronic chagasic patients, triatomines and sylvatic reservoirs from Paraná state, Southern Brazil, were studied using the randomly amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored polymerase chain reaction amplification (SSR-PCR) techniques. Sylvio and Esmeraldo stocks were used as reference for T. cruzi I and II, respectively. The DNA was amplified using three different primers for RAPD and (CA)8RY primer for SSRPCR. Gel analyses were scored by eye for a numerical taxonomy analysis based on the proportion of shared bands and by employing computer programs. The phenograms showed the existence of two distinct genetic groups: one containing the strains from chronic chagasic patients grouped with Esmeraldo and the other with the strains from triatomines and sylvatic reservoirs grouped with Sylvio. The existence of two phylogenetic groups of T. cruzi in Paraná state, Southern Brazil, is described for the first time. 2007 artículo científico 1679-9291 https://www.redalyc.org/articulo.oa?id=307226620004 en http://www.redalyc.org/revista.oa?id=3072 Acta Scientiarum. Health Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Health Sciences (Brasil) Num.1 Vol.29 |
| title | Genetic diversity of Trypanosoma cruzi natural populations in Paraná state, southern Brazil |
| topic | Medicina SSR PCR RAPD genetic groups Trypanosoma cruzi |
| url | https://www.redalyc.org/articulo.oa?id=307226620004 |