Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidade Estadual de Maringá
2015
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| _version_ | 1876449429894987776 |
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| author | Genilton da Silva Faheina Junior |
| author_facet | Genilton da Silva Faheina Junior |
| contents | Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome Genilton da Silva Faheina Junior Mariza Vieira da Fonseca Sabóia Amorim Caroline Gondim de Souza Diego Menezes de Sousa Kally Alves de Sousa Gustavo Adolfo Saavedra Pinto Medicina enzyme induction Cellulolytic enzymes Studies on new microbial sources of cellulas e and accurate assessment of the steps that increase cellulase production are e ssential strategies to reduce cost s of various processes using such enzymes. This study aimed at the selection of cellulase-producing filamentous fungi, and at the research of parameters involving cellulase production by submerge d fermentation. The first test consisted of selecting the best cellulase-producing microor ganisms (FPase) in Erlenmeyer flas ks containing 200 mL of specific growth medium. The next test was designed to furthe r investigate the enzyme production in fermentation with four types of soluble sugars: glucose, lactose, su crose and xylose. In bioreact or tests, three different inoculation strategies were analyzed. The best FPase activity was presented by the strain Trichoderma sp. CMIAT 041 (49.9 FPU L -1 ) and CMCase by the fungus Lasiodiplodia theobromae CMIAT 096 (350.0 U L -1 ). Sucrose proved to be the best option among the solubl e sugars tested, with higher rates of FPase activity (49.9 FPU L -1 ) and CMCase (119.7 U L -1 ). The best inoculation strategy for the bioreactor was a spore suspension obtained from a semi-solid st ate fermentation of wheat bran for 72h. 2015 editorial 1679-9291 https://www.redalyc.org/articulo.oa?id=307239651003 en http://www.redalyc.org/revista.oa?id=3072 Acta Scientiarum. Health Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Health Sciences (Brasil) Num.1 Vol.37 |
| format | Artículo científico |
| id | redalyc_307239651003 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome Genilton da Silva Faheina Junior Medicina enzyme induction Cellulolytic enzymes Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome Genilton da Silva Faheina Junior Mariza Vieira da Fonseca Sabóia Amorim Caroline Gondim de Souza Diego Menezes de Sousa Kally Alves de Sousa Gustavo Adolfo Saavedra Pinto Medicina enzyme induction Cellulolytic enzymes Studies on new microbial sources of cellulas e and accurate assessment of the steps that increase cellulase production are e ssential strategies to reduce cost s of various processes using such enzymes. This study aimed at the selection of cellulase-producing filamentous fungi, and at the research of parameters involving cellulase production by submerge d fermentation. The first test consisted of selecting the best cellulase-producing microor ganisms (FPase) in Erlenmeyer flas ks containing 200 mL of specific growth medium. The next test was designed to furthe r investigate the enzyme production in fermentation with four types of soluble sugars: glucose, lactose, su crose and xylose. In bioreact or tests, three different inoculation strategies were analyzed. The best FPase activity was presented by the strain Trichoderma sp. CMIAT 041 (49.9 FPU L -1 ) and CMCase by the fungus Lasiodiplodia theobromae CMIAT 096 (350.0 U L -1 ). Sucrose proved to be the best option among the solubl e sugars tested, with higher rates of FPase activity (49.9 FPU L -1 ) and CMCase (119.7 U L -1 ). The best inoculation strategy for the bioreactor was a spore suspension obtained from a semi-solid st ate fermentation of wheat bran for 72h. 2015 editorial 1679-9291 https://www.redalyc.org/articulo.oa?id=307239651003 en http://www.redalyc.org/revista.oa?id=3072 Acta Scientiarum. Health Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Health Sciences (Brasil) Num.1 Vol.37 |
| title | Strategies to increase cellulase production with submerged fermentation using fungi isolated from the Brazilian biome |
| topic | Medicina enzyme induction Cellulolytic enzymes |
| url | https://www.redalyc.org/articulo.oa?id=307239651003 |