Production of fibrinogenolytic and fibrinolytic enzymes by a strain of Penicillium sp. isolated from contaminated soil with industrial effluent
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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual de Maringá
2019
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| _version_ | 1876435375980806144 |
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| author | Lillian Maria Baggio |
| author_facet | Lillian Maria Baggio |
| contents | Production of fibrinogenolytic and fibrinolytic enzymes by a strain of Penicillium sp. isolated from contaminated soil with industrial effluent Lillian Maria Baggio Luciano Aparecido Panagio Fabiana Guillen Moreira Gasparin Daniele Sartori Maria Antonia Pedrine Colabone Celligoi Cristiani Baldo Medicina fungi Thrombosis Penicillium fibrin(geno)lytic enzymes Cardiovascular diseases associated with thrombosis are one of the main causes of death all around the world. Urokinase, streptokinase, and tissue plasminogen activator are the major thrombolytic agents used to treat thrombosis. However, the fact that these agents have several side effects and high prices has driven the search for safer and more economically viable compounds for the treatment of cardiovascular diseases. Thus, the aim of this study was to evaluate the potential of fungi isolated from industrial effluents to produce fibrino(geno)lytic enzymes. The selection of the protease-producing strains showed that only the BF20 strain was able to produce proteolytic halos in milk agar. This strain identified as belonging to the genus Penicillium was cultivated in submerged fermentation. Different media composition were tested to evaluate proteolytic activity, and the results showed that the medium containing 0.1% yeast extract and 1% skim milk, pH 5.0, present higher azocaseinolytic activity (0.24 U mL-1 min.-1). This sample also showed the ability to degrade fibrinogen and fibrin after 15 and 120 min. of incubation, respectively. These results indicate that the BF20 strain has a thrombolytic potential, effectively degrading fibrinogen and fibrin, having great application in the health area. 2019 artículo científico 1679-9291 https://www.redalyc.org/articulo.oa?id=307261031006 https://www.redalyc.org/journal/3072/307261031006/ https://www.redalyc.org/journal/3072/307261031006/html/ https://www.redalyc.org/journal/3072/307261031006/307261031006.epub https://www.redalyc.org/journal/3072/307261031006/movil https://doi.org/10.4025/actascihealthsci.v41i1.40606 en http://www.redalyc.org/revista.oa?id=3072 Acta Scientiarum. Health Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Health Sciences (Brasil) Vol.41 |
| format | Artículo científico |
| id | redalyc_307261031006 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Production of fibrinogenolytic and fibrinolytic enzymes by a strain of Penicillium sp. isolated from contaminated soil with industrial effluent Lillian Maria Baggio Medicina fungi Thrombosis Penicillium fibrin(geno)lytic enzymes Production of fibrinogenolytic and fibrinolytic enzymes by a strain of Penicillium sp. isolated from contaminated soil with industrial effluent Lillian Maria Baggio Luciano Aparecido Panagio Fabiana Guillen Moreira Gasparin Daniele Sartori Maria Antonia Pedrine Colabone Celligoi Cristiani Baldo Medicina fungi Thrombosis Penicillium fibrin(geno)lytic enzymes Cardiovascular diseases associated with thrombosis are one of the main causes of death all around the world. Urokinase, streptokinase, and tissue plasminogen activator are the major thrombolytic agents used to treat thrombosis. However, the fact that these agents have several side effects and high prices has driven the search for safer and more economically viable compounds for the treatment of cardiovascular diseases. Thus, the aim of this study was to evaluate the potential of fungi isolated from industrial effluents to produce fibrino(geno)lytic enzymes. The selection of the protease-producing strains showed that only the BF20 strain was able to produce proteolytic halos in milk agar. This strain identified as belonging to the genus Penicillium was cultivated in submerged fermentation. Different media composition were tested to evaluate proteolytic activity, and the results showed that the medium containing 0.1% yeast extract and 1% skim milk, pH 5.0, present higher azocaseinolytic activity (0.24 U mL-1 min.-1). This sample also showed the ability to degrade fibrinogen and fibrin after 15 and 120 min. of incubation, respectively. These results indicate that the BF20 strain has a thrombolytic potential, effectively degrading fibrinogen and fibrin, having great application in the health area. 2019 artículo científico 1679-9291 https://www.redalyc.org/articulo.oa?id=307261031006 https://www.redalyc.org/journal/3072/307261031006/ https://www.redalyc.org/journal/3072/307261031006/html/ https://www.redalyc.org/journal/3072/307261031006/307261031006.epub https://www.redalyc.org/journal/3072/307261031006/movil https://doi.org/10.4025/actascihealthsci.v41i1.40606 en http://www.redalyc.org/revista.oa?id=3072 Acta Scientiarum. Health Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Health Sciences (Brasil) Vol.41 |
| title | Production of fibrinogenolytic and fibrinolytic enzymes by a strain of Penicillium sp. isolated from contaminated soil with industrial effluent |
| topic | Medicina fungi Thrombosis Penicillium fibrin(geno)lytic enzymes |
| url | https://www.redalyc.org/articulo.oa?id=307261031006 https://www.redalyc.org/journal/3072/307261031006/ https://www.redalyc.org/journal/3072/307261031006/html/ https://www.redalyc.org/journal/3072/307261031006/307261031006.epub https://www.redalyc.org/journal/3072/307261031006/movil https://doi.org/10.4025/actascihealthsci.v41i1.40606 |