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1. Verfasser: Lamiaa Elsayed Mokhtar Deef
Format: Artículo científico
Sprache:en
Veröffentlicht: Universidade Estadual de Maringá 2023
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https://www.redalyc.org/journal/3072/307276195022/
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https://www.redalyc.org/journal/3072/307276195022/307276195022.epub
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https://doi.org/10.4025/actascihealthsci.v45i1.61090
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author Lamiaa Elsayed Mokhtar Deef
author_facet Lamiaa Elsayed Mokhtar Deef
contents Estimation of antigenotoxic properties of Ginger (Zingibe officinale) for DNA Damage after Exposure to Patulin in rats Lamiaa Elsayed Mokhtar Deef kadry Abd-El kader El-Bakry Lotfy Zakaria Habbak Samia Salem El-Naeli Medicina ginger Patulin genotoxicity alkaline comet assay antioxidant DNA damage Patulin, a known fungal poison, is an important pollutant in apples and products derived from apples and fodder. Ginger improved the liver function and prevents hepatotoxicity against many toxins and used in treatment of various diseases. The genetic toxicity of patulin in the liver of rats using the comet assay in vivo was investigated and effect of ginger (Zingibe officinale) on DNA damage induced by patulin. Five groups of adult male Sprague Dawley rats were used. The rats were given 3.75 mg kg-1 intraperitoneal (i.p) patulin. The rats were treated with ginger at a dose (100 mg kg-1) for four weeks and eight weeks. DNA damage was measured in the liver of rats using a standard genetic toxicity tests comet assay. Tail length and percentage of tail DNA within the comet increased significantly (p <0.05) in patulin group (p =0.01), (p =0.000) respectively, compared with control group a time-dependent manner, indicating an increase in DNA damage. The liver tissue showed signs of improvement in DNA damage after treating rats with ginger. In conclusion, these results indicate that patulin is genotoxic at the injected dose and that liver is an important target organ. Instead, ginger therapy can reduce DNA damage in liver cells from the toxicity caused by patulin. 2023 artículo científico 1679-9291 https://www.redalyc.org/articulo.oa?id=307276195022 https://www.redalyc.org/journal/3072/307276195022/ https://www.redalyc.org/journal/3072/307276195022/html/ https://www.redalyc.org/journal/3072/307276195022/307276195022.epub https://www.redalyc.org/journal/3072/307276195022/movil https://doi.org/10.4025/actascihealthsci.v45i1.61090 en http://www.redalyc.org/revista.oa?id=3072 Acta Scientiarum. Health Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Health Sciences (Brasil) Vol.45
format Artículo científico
id redalyc_307276195022
language en
publishDate 2023
publisher Universidade Estadual de Maringá
spellingShingle Estimation of antigenotoxic properties of Ginger (Zingibe officinale) for DNA Damage after Exposure to Patulin in rats
Lamiaa Elsayed Mokhtar Deef
Medicina
ginger
Patulin
genotoxicity
alkaline comet assay
antioxidant DNA damage
Estimation of antigenotoxic properties of Ginger (Zingibe officinale) for DNA Damage after Exposure to Patulin in rats Lamiaa Elsayed Mokhtar Deef kadry Abd-El kader El-Bakry Lotfy Zakaria Habbak Samia Salem El-Naeli Medicina ginger Patulin genotoxicity alkaline comet assay antioxidant DNA damage Patulin, a known fungal poison, is an important pollutant in apples and products derived from apples and fodder. Ginger improved the liver function and prevents hepatotoxicity against many toxins and used in treatment of various diseases. The genetic toxicity of patulin in the liver of rats using the comet assay in vivo was investigated and effect of ginger (Zingibe officinale) on DNA damage induced by patulin. Five groups of adult male Sprague Dawley rats were used. The rats were given 3.75 mg kg-1 intraperitoneal (i.p) patulin. The rats were treated with ginger at a dose (100 mg kg-1) for four weeks and eight weeks. DNA damage was measured in the liver of rats using a standard genetic toxicity tests comet assay. Tail length and percentage of tail DNA within the comet increased significantly (p <0.05) in patulin group (p =0.01), (p =0.000) respectively, compared with control group a time-dependent manner, indicating an increase in DNA damage. The liver tissue showed signs of improvement in DNA damage after treating rats with ginger. In conclusion, these results indicate that patulin is genotoxic at the injected dose and that liver is an important target organ. Instead, ginger therapy can reduce DNA damage in liver cells from the toxicity caused by patulin. 2023 artículo científico 1679-9291 https://www.redalyc.org/articulo.oa?id=307276195022 https://www.redalyc.org/journal/3072/307276195022/ https://www.redalyc.org/journal/3072/307276195022/html/ https://www.redalyc.org/journal/3072/307276195022/307276195022.epub https://www.redalyc.org/journal/3072/307276195022/movil https://doi.org/10.4025/actascihealthsci.v45i1.61090 en http://www.redalyc.org/revista.oa?id=3072 Acta Scientiarum. Health Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Health Sciences (Brasil) Vol.45
title Estimation of antigenotoxic properties of Ginger (Zingibe officinale) for DNA Damage after Exposure to Patulin in rats
topic Medicina
ginger
Patulin
genotoxicity
alkaline comet assay
antioxidant DNA damage
url https://www.redalyc.org/articulo.oa?id=307276195022
https://www.redalyc.org/journal/3072/307276195022/
https://www.redalyc.org/journal/3072/307276195022/html/
https://www.redalyc.org/journal/3072/307276195022/307276195022.epub
https://www.redalyc.org/journal/3072/307276195022/movil
https://doi.org/10.4025/actascihealthsci.v45i1.61090