COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad Nacional de Colombia
2015
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| _version_ | 1876423815764901888 |
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| author | Alejandra MUÑOZ BODNAR |
| author_facet | Alejandra MUÑOZ BODNAR |
| contents | COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS Alejandra MUÑOZ BODNAR Leidy Mariel CRUZ GÓMEZ Adriana BERNAL Boris SZUREK Camilo Ernesto LÓPEZ CARRASCAL Biología cassava cassava cassava cassava cassava Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB), a major disease for cassava crops in South America and Africa. Until now the development of the disease is measured via AUDPC (Area Under Disease Progress Curve) but no reliable quantitative methods are available probably due to high variability of bacterial growth in planta. To establish an accurate method for bacterial quantification during the course of Xam infection within the host tissues, we analyzed bacterial populations upon stem and leaf-puncturing as well as leaf-clipping of cassava varieties MCOL1522 and SG107-35 challenged with the virulent Xam strain CIO151. Here, we show that the movement of bacteria along the tissues and especially in leaves is stochastic. Moreover, we were able to demonstrate differential growth of virulent Xam strain CIO151 upon stem-puncturing and quantification of bacteria 6 cm. away from the inoculation point of two varieties displaying contrasting levels of susceptibility. 2015 artículo científico 0120-548X https://www.redalyc.org/articulo.oa?id=319038639005 en http://www.redalyc.org/revista.oa?id=3190 Acta Biológica Colombiana application/pdf Universidad Nacional de Colombia Acta Biológica Colombiana (Colombia) Num.2 Vol.20 |
| format | Artículo científico |
| id | redalyc_319038639005 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS Alejandra MUÑOZ BODNAR Biología cassava cassava cassava cassava cassava COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS Alejandra MUÑOZ BODNAR Leidy Mariel CRUZ GÓMEZ Adriana BERNAL Boris SZUREK Camilo Ernesto LÓPEZ CARRASCAL Biología cassava cassava cassava cassava cassava Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB), a major disease for cassava crops in South America and Africa. Until now the development of the disease is measured via AUDPC (Area Under Disease Progress Curve) but no reliable quantitative methods are available probably due to high variability of bacterial growth in planta. To establish an accurate method for bacterial quantification during the course of Xam infection within the host tissues, we analyzed bacterial populations upon stem and leaf-puncturing as well as leaf-clipping of cassava varieties MCOL1522 and SG107-35 challenged with the virulent Xam strain CIO151. Here, we show that the movement of bacteria along the tissues and especially in leaves is stochastic. Moreover, we were able to demonstrate differential growth of virulent Xam strain CIO151 upon stem-puncturing and quantification of bacteria 6 cm. away from the inoculation point of two varieties displaying contrasting levels of susceptibility. 2015 artículo científico 0120-548X https://www.redalyc.org/articulo.oa?id=319038639005 en http://www.redalyc.org/revista.oa?id=3190 Acta Biológica Colombiana application/pdf Universidad Nacional de Colombia Acta Biológica Colombiana (Colombia) Num.2 Vol.20 |
| title | COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS |
| topic | Biología cassava cassava cassava cassava cassava |
| url | https://www.redalyc.org/articulo.oa?id=319038639005 |