Olive oil as an alternative to boar semen cryopreservation
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| Format: | Artículo científico |
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Universidad CES
2016
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| author | Estela Ferandes e Silva |
| author_facet | Estela Ferandes e Silva |
| contents | Olive oil as an alternative to boar semen cryopreservation Estela Ferandes e Silva Tainã Figueiredo Cardoso Fabiana Lemos Goulart Dutra Antonio Sergio Varela Junior Fábio Pereira Leivas Leite Carine Dahl Corcini Veterinaria Antioxidants in vitro penetration trial mitochondrial functionality The aim of this study was to evaluate different concentrations of olive oil for cryopreservation of boar semen. A total of 21 eyaculates of 18 males with motility ≥70% were used. For freezing, the semen was diluted in treatments: control (Cont–egg yolk), “Koroneiki” oil in egg yolk at 0.25% (A025), 0.50% (A050), 0.75% (A075) and 1.0% (A10). Straws with 5.10 7 sperm/ml were frozen and stored in liquid nitrogen. Thawing was done at 37ºC for 30 seconds and motility, mitochondrial functionality, DNA integrity, oocite penetration rate and the number of sperm per oocite (in the in vitro penetration trial) were evaluated. The variables were compared using the Kruskal-Wallis test. Even though no statistical difference was found between control and treatments containingolive oil (p>0.05), the treatment with 0.25% oil concentration showed tendences towards sperm protection, outperforming the controls in mitochondrial functionality and preservation of the fertilizing capabilities in the in vitro penetration trial. This is why, olive oil could represent an alternative to help cryopreservation of boar semen. 2016 artículo científico 1900-9607 https://www.redalyc.org/articulo.oa?id=321445731002 en http://www.redalyc.org/revista.oa?id=3214 Revista CES Medicina Veterinaria y Zootecnia application/pdf Universidad CES Revista CES Medicina Veterinaria y Zootecnia (Colombia) Num.1 Vol.11 |
| format | Artículo científico |
| id | redalyc_321445731002 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Universidad CES |
| spellingShingle | Olive oil as an alternative to boar semen cryopreservation Estela Ferandes e Silva Veterinaria Antioxidants in vitro penetration trial mitochondrial functionality Olive oil as an alternative to boar semen cryopreservation Estela Ferandes e Silva Tainã Figueiredo Cardoso Fabiana Lemos Goulart Dutra Antonio Sergio Varela Junior Fábio Pereira Leivas Leite Carine Dahl Corcini Veterinaria Antioxidants in vitro penetration trial mitochondrial functionality The aim of this study was to evaluate different concentrations of olive oil for cryopreservation of boar semen. A total of 21 eyaculates of 18 males with motility ≥70% were used. For freezing, the semen was diluted in treatments: control (Cont–egg yolk), “Koroneiki” oil in egg yolk at 0.25% (A025), 0.50% (A050), 0.75% (A075) and 1.0% (A10). Straws with 5.10 7 sperm/ml were frozen and stored in liquid nitrogen. Thawing was done at 37ºC for 30 seconds and motility, mitochondrial functionality, DNA integrity, oocite penetration rate and the number of sperm per oocite (in the in vitro penetration trial) were evaluated. The variables were compared using the Kruskal-Wallis test. Even though no statistical difference was found between control and treatments containingolive oil (p>0.05), the treatment with 0.25% oil concentration showed tendences towards sperm protection, outperforming the controls in mitochondrial functionality and preservation of the fertilizing capabilities in the in vitro penetration trial. This is why, olive oil could represent an alternative to help cryopreservation of boar semen. 2016 artículo científico 1900-9607 https://www.redalyc.org/articulo.oa?id=321445731002 en http://www.redalyc.org/revista.oa?id=3214 Revista CES Medicina Veterinaria y Zootecnia application/pdf Universidad CES Revista CES Medicina Veterinaria y Zootecnia (Colombia) Num.1 Vol.11 |
| title | Olive oil as an alternative to boar semen cryopreservation |
| topic | Veterinaria Antioxidants in vitro penetration trial mitochondrial functionality |
| url | https://www.redalyc.org/articulo.oa?id=321445731002 |