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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2012
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| Online Access: | https://www.redalyc.org/articulo.oa?id=32721622014 |
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| _version_ | 1866814186201284608 |
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| author | Virgínia F. Braga |
| author_facet | Virgínia F. Braga |
| contents | Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae) Virgínia F. Braga Giselle C. Mendes Raphael T.R. Oliveira Carla Q.G. Soares Cristiano F. Resende Leandro C. Pinto Reinaldo de Santana Lyderson F. Viccini Nádia R.B. Raposo Paulo H.P. Peixoto Multidisciplinaria (Ciencias Naturales y Exactas) Tissue culture natural products biological effects biodiversity conservation This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC50 = 169.3 µg mL-1. HF showed a non-dose dependent analgesic activity in the writhing test; its antinociceptive activity in the hot plate test was restricted to 500 mg kg-1, which is the highest dose. The results of this study showed the potential of tissue culture on conservation and large scale multiplication and confirmed the traditional folk medicine use of V. litoralis. 2012 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32721622014 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.84 |
| format | Artículo científico |
| id | redalyc_32721622014 |
| language | en |
| publishDate | 2012 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae) Virgínia F. Braga Multidisciplinaria (Ciencias Naturales y Exactas) Tissue culture natural products biological effects biodiversity conservation Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae) Virgínia F. Braga Giselle C. Mendes Raphael T.R. Oliveira Carla Q.G. Soares Cristiano F. Resende Leandro C. Pinto Reinaldo de Santana Lyderson F. Viccini Nádia R.B. Raposo Paulo H.P. Peixoto Multidisciplinaria (Ciencias Naturales y Exactas) Tissue culture natural products biological effects biodiversity conservation This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC50 = 169.3 µg mL-1. HF showed a non-dose dependent analgesic activity in the writhing test; its antinociceptive activity in the hot plate test was restricted to 500 mg kg-1, which is the highest dose. The results of this study showed the potential of tissue culture on conservation and large scale multiplication and confirmed the traditional folk medicine use of V. litoralis. 2012 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32721622014 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.84 |
| title | Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae) |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) Tissue culture natural products biological effects biodiversity conservation |
| url | https://www.redalyc.org/articulo.oa?id=32721622014 |