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Main Author:	Fernanda A. Alves-Costa
Format:	Artículo científico
Language:	en
Published:	Academia Brasileira de Ciências 2012
Subjects:	Multidisciplinaria (Ciencias Naturales y Exactas) GH zebrafish transgenic growth hormone Differential display
Online Access:	https://www.redalyc.org/articulo.oa?id=32722628021
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author	Fernanda A. Alves-Costa
author_facet	Fernanda A. Alves-Costa
contents	A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach Fernanda A. Alves-Costa Márcio A. Figueiredo Carlos F.C. Lanes Daniela V. Almeida Luis F. Marins Adriane P. Wasko Multidisciplinaria (Ciencias Naturales y Exactas) GH zebrafish transgenic growth hormone Differential display The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating levels of this hormone are found in zebrafishes. 2012 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32722628021 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.2 Vol.84
format	Artículo científico
id	redalyc_32722628021
language	en
publishDate	2012
publisher	Academia Brasileira de Ciências
spellingShingle	A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach Fernanda A. Alves-Costa Multidisciplinaria (Ciencias Naturales y Exactas) GH zebrafish transgenic growth hormone Differential display A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach Fernanda A. Alves-Costa Márcio A. Figueiredo Carlos F.C. Lanes Daniela V. Almeida Luis F. Marins Adriane P. Wasko Multidisciplinaria (Ciencias Naturales y Exactas) GH zebrafish transgenic growth hormone Differential display The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating levels of this hormone are found in zebrafishes. 2012 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32722628021 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.2 Vol.84
title	A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach
topic	Multidisciplinaria (Ciencias Naturales y Exactas) GH zebrafish transgenic growth hormone Differential display
url	https://www.redalyc.org/articulo.oa?id=32722628021

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