Production, characterization and application of inulinase from fungal endophyte CCMB 328
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| Natura: | Artículo científico |
| Lingua: | en |
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Academia Brasileira de Ciências
2012
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| _version_ | 1876445589906915328 |
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| author | Diego S. Nascimento |
| author_facet | Diego S. Nascimento |
| contents | Production, characterization and application of inulinase from fungal endophyte CCMB 328 Diego S. Nascimento Gildomar Valasques Junior Pedro Fernandes Geise C.A. Ribeiro Danyo M. Lima Aristóteles Góes-Neto Rodrigo Q. Oliveira Rita de Cassia L. Figueiredo-Ribeiro Sandra A. de Assis Multidisciplinaria (Ciencias Naturales y Exactas) semi arid Inulin yeasts inulinase Inulinase (B;-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the B;-2,1 linkage of inulin, a polyfructan consisting of linear B;-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v). 2012 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32722628023 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.2 Vol.84 |
| format | Artículo científico |
| id | redalyc_32722628023 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Production, characterization and application of inulinase from fungal endophyte CCMB 328 Diego S. Nascimento Multidisciplinaria (Ciencias Naturales y Exactas) semi arid Inulin yeasts inulinase Production, characterization and application of inulinase from fungal endophyte CCMB 328 Diego S. Nascimento Gildomar Valasques Junior Pedro Fernandes Geise C.A. Ribeiro Danyo M. Lima Aristóteles Góes-Neto Rodrigo Q. Oliveira Rita de Cassia L. Figueiredo-Ribeiro Sandra A. de Assis Multidisciplinaria (Ciencias Naturales y Exactas) semi arid Inulin yeasts inulinase Inulinase (B;-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the B;-2,1 linkage of inulin, a polyfructan consisting of linear B;-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v). 2012 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32722628023 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.2 Vol.84 |
| title | Production, characterization and application of inulinase from fungal endophyte CCMB 328 |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) semi arid Inulin yeasts inulinase |
| url | https://www.redalyc.org/articulo.oa?id=32722628023 |