Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
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| Format: | Artículo científico |
| Sprache: | en |
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Academia Brasileira de Ciências
2013
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| author | SUZAN K.V. BERTOLUCCI |
| author_facet | SUZAN K.V. BERTOLUCCI |
| contents | Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata SUZAN K.V. BERTOLUCCI ANA B.D. PEREIRA JOSÉ E.B.P. PINTO ALAÍDE B. OLIVEIRA FERNÃO C. BRAGA Multidisciplinaria (Ciencias Naturales y Exactas) HPLC post storage harvest DAD analysis The leaves of Mikania laevigata and Mikania glomerata are used in Brazil to treat respiratory affections, being kaurane-type diterpenes and coumarin considered as the bioactive compounds. The present study reports an investigation on the HPLC-DAD profi les and contents of coumarin ( 1 ), trans-o -coumaric ( 2 ), kaurenoic ( 3 ), benzoylgrandifl oric ( 4 ) and cinnamoylgrandifl oric ( 5 ) acids in dried leaves of Mikania species stored in dark room under controlled conditions. Excepting 2 , the constituents were isolated and purifi ed to be employed as reference compounds. The samples were analyzed at three monthly intervals up to 18 months for M. laevigata and 12 months for M. glomerata . trans-o -Coumaric was not detected in both, whereas 1 occurred only in M. laevigata . The concentrations of the assayed constituents did not vary signifi cantly within the evaluated period ( p vary signifi cantly within the evaluated period ( p vary signifi cantly within the evaluated period ( < 0.05), for both species. In contrast, changes in the chromatographic profi les and spectral purity of peaks from 3 , 4 and 5 were detected in samples of both Mikania stored for three months, while the coumarin profi le in M. laevigata modifi ed after six months of storage. The evaluation of chromatographic profi les based on spectral purity analyses of selected peaks was shown to be a more robust tool to access chemical stability of Mikania samples than the quantitation of chemical markers’ contents. 2013 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32727844004 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.2 Vol.85 |
| format | Artículo científico |
| id | redalyc_32727844004 |
| institution | Redalyc |
| language | en |
| publishDate | 2013 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata SUZAN K.V. BERTOLUCCI Multidisciplinaria (Ciencias Naturales y Exactas) HPLC post storage harvest DAD analysis Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata SUZAN K.V. BERTOLUCCI ANA B.D. PEREIRA JOSÉ E.B.P. PINTO ALAÍDE B. OLIVEIRA FERNÃO C. BRAGA Multidisciplinaria (Ciencias Naturales y Exactas) HPLC post storage harvest DAD analysis The leaves of Mikania laevigata and Mikania glomerata are used in Brazil to treat respiratory affections, being kaurane-type diterpenes and coumarin considered as the bioactive compounds. The present study reports an investigation on the HPLC-DAD profi les and contents of coumarin ( 1 ), trans-o -coumaric ( 2 ), kaurenoic ( 3 ), benzoylgrandifl oric ( 4 ) and cinnamoylgrandifl oric ( 5 ) acids in dried leaves of Mikania species stored in dark room under controlled conditions. Excepting 2 , the constituents were isolated and purifi ed to be employed as reference compounds. The samples were analyzed at three monthly intervals up to 18 months for M. laevigata and 12 months for M. glomerata . trans-o -Coumaric was not detected in both, whereas 1 occurred only in M. laevigata . The concentrations of the assayed constituents did not vary signifi cantly within the evaluated period ( p vary signifi cantly within the evaluated period ( p vary signifi cantly within the evaluated period ( < 0.05), for both species. In contrast, changes in the chromatographic profi les and spectral purity of peaks from 3 , 4 and 5 were detected in samples of both Mikania stored for three months, while the coumarin profi le in M. laevigata modifi ed after six months of storage. The evaluation of chromatographic profi les based on spectral purity analyses of selected peaks was shown to be a more robust tool to access chemical stability of Mikania samples than the quantitation of chemical markers’ contents. 2013 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32727844004 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.2 Vol.85 |
| title | Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) HPLC post storage harvest DAD analysis |
| url | https://www.redalyc.org/articulo.oa?id=32727844004 |