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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2013
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| Online Access: | https://www.redalyc.org/articulo.oa?id=32729375006 |
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| _version_ | 1866812402287247360 |
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| author | JOSIANE C. CLAUDINO |
| author_facet | JOSIANE C. CLAUDINO |
| contents | Evaluation of morpho-anatomical and chemical differences between varieties of the medicinal plant Casearia sylvestris Swartz JOSIANE C. CLAUDINO LUIS V.S. DO SACRAMENTO INGRID KOCH HELEN A. SANTOS ALBERTO J. CAVALHEIRO ARISTEU G. TININIS ANDRÉ G. DOS SANTOS Multidisciplinaria (Ciencias Naturales y Exactas) morpho anatomy diterpenes chromatography Casearia sylvestris Casearia sylvestris Swartz (Salicaceae) has been used in traditional medicine and its leaf extracts have been exhibited important pharmacological activities. The species presents morphological, chemical and genetic variation. Two varieties are considered due external morphological differences: C. sylvestris var. sylvestris and var. lingua . There are difficulties in definition of these varieties. The objective of this work is to evaluate chemical and morpho-anatomical differences between C. sylvestris varieties that can be applied in their distinction for pharmaceutical or botanical purposes. Transverse and paradermic sections of leaves were prepared for morpho- anatomical, histochemical and quantitative microscopy (stomatal and palisade index) analyses. Diterpene profiles of the specimens were obtained by HPLC-DAD and TLC. Morpho-anatomical analyses demonstrated significant differences between the varieties only in paradermic sections: var. sylvestris - polygonal epidermic cell walls and hypostomatic; var. lingua - rounded epidermic cell walls and amphistomatic. No differences were observed for stomatal index; palisade index was found 2.8 for var. lingu a and 3.9 for var. sylvestris . Chromatographic analyses confirmed previous results demonstrating that diterpene profile in varieties differs, with predominance of these metabolites in var. sylvestris . In conclusion, this work indicates that chromatographic analysis besides morpho- anatomical analysis can be applied in distinction of C. sylvestris varieties. 2013 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32729375006 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.85 |
| format | Artículo científico |
| id | redalyc_32729375006 |
| language | en |
| publishDate | 2013 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Evaluation of morpho-anatomical and chemical differences between varieties of the medicinal plant Casearia sylvestris Swartz JOSIANE C. CLAUDINO Multidisciplinaria (Ciencias Naturales y Exactas) morpho anatomy diterpenes chromatography Casearia sylvestris Evaluation of morpho-anatomical and chemical differences between varieties of the medicinal plant Casearia sylvestris Swartz JOSIANE C. CLAUDINO LUIS V.S. DO SACRAMENTO INGRID KOCH HELEN A. SANTOS ALBERTO J. CAVALHEIRO ARISTEU G. TININIS ANDRÉ G. DOS SANTOS Multidisciplinaria (Ciencias Naturales y Exactas) morpho anatomy diterpenes chromatography Casearia sylvestris Casearia sylvestris Swartz (Salicaceae) has been used in traditional medicine and its leaf extracts have been exhibited important pharmacological activities. The species presents morphological, chemical and genetic variation. Two varieties are considered due external morphological differences: C. sylvestris var. sylvestris and var. lingua . There are difficulties in definition of these varieties. The objective of this work is to evaluate chemical and morpho-anatomical differences between C. sylvestris varieties that can be applied in their distinction for pharmaceutical or botanical purposes. Transverse and paradermic sections of leaves were prepared for morpho- anatomical, histochemical and quantitative microscopy (stomatal and palisade index) analyses. Diterpene profiles of the specimens were obtained by HPLC-DAD and TLC. Morpho-anatomical analyses demonstrated significant differences between the varieties only in paradermic sections: var. sylvestris - polygonal epidermic cell walls and hypostomatic; var. lingua - rounded epidermic cell walls and amphistomatic. No differences were observed for stomatal index; palisade index was found 2.8 for var. lingu a and 3.9 for var. sylvestris . Chromatographic analyses confirmed previous results demonstrating that diterpene profile in varieties differs, with predominance of these metabolites in var. sylvestris . In conclusion, this work indicates that chromatographic analysis besides morpho- anatomical analysis can be applied in distinction of C. sylvestris varieties. 2013 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32729375006 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.85 |
| title | Evaluation of morpho-anatomical and chemical differences between varieties of the medicinal plant Casearia sylvestris Swartz |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) morpho anatomy diterpenes chromatography Casearia sylvestris |
| url | https://www.redalyc.org/articulo.oa?id=32729375006 |