Standardization of a method to study angiogenesis in a mouse model
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| Natura: | Artículo científico |
| Lingua: | en |
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Academia Brasileira de Ciências
2013
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| _version_ | 1876449417184149504 |
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| author | DAVID FEDER |
| author_facet | DAVID FEDER |
| contents | Standardization of a method to study angiogenesis in a mouse model DAVID FEDER FABIO F. PERRAZO EDIMAR C. PEREIRA SILVANA FORSAIT CECÍLIA K.R. FEDER PAULO E.B. JUNQUEIRA VIRGINIA B.C. JUNQUEIRA LIGIA A. AZZALIS FERNANDO L.A. FONSECA Multidisciplinaria (Ciencias Naturales y Exactas) thalidomide sponge implant Angiogenesis model hemoglobin measurement In the adult organism, angiogenesis is restricted to a few physiological conditions. On the other hand, uncontrolled angiogenesis have often been associated to angiogenesis-dependent pathologies. A variety of animal models have been described to provide more quantitative analysis of in vivo angiogenesis and to characterize pro- and antiangiogenic molecules. However, it is still necessary to establish a quantitative, reproducible and specific method for studies of angiogenesis factors and inhibitors. This work aimed to standardize a method for the study of angiogenesis and to investigate the effects of thalidomide on angiogenesis. Sponges of 0.5 x 0.5 x 0.5 cm were implanted in the back of mice groups, control and experimental (thalidomide 200 mg/K/day by gavage). After seven days, the sponges were removed. The dosage of hemoglobin in sponge and in circulation was performed and the ratio between the values was tested using nonparametric Mann-Whitney test. Results have shown that sponge-induced angiogenesis quantitated by ratio between hemoglobin content in serum and in sponge is a helpful model for in vivo studies on angiogenesis. Moreover, it was observed that sponge-induced angiogenesis can be suppressed by thalidomide, corroborating to the validity of the standardized method. 2013 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32729375026 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.85 |
| format | Artículo científico |
| id | redalyc_32729375026 |
| institution | Redalyc |
| language | en |
| publishDate | 2013 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Standardization of a method to study angiogenesis in a mouse model DAVID FEDER Multidisciplinaria (Ciencias Naturales y Exactas) thalidomide sponge implant Angiogenesis model hemoglobin measurement Standardization of a method to study angiogenesis in a mouse model DAVID FEDER FABIO F. PERRAZO EDIMAR C. PEREIRA SILVANA FORSAIT CECÍLIA K.R. FEDER PAULO E.B. JUNQUEIRA VIRGINIA B.C. JUNQUEIRA LIGIA A. AZZALIS FERNANDO L.A. FONSECA Multidisciplinaria (Ciencias Naturales y Exactas) thalidomide sponge implant Angiogenesis model hemoglobin measurement In the adult organism, angiogenesis is restricted to a few physiological conditions. On the other hand, uncontrolled angiogenesis have often been associated to angiogenesis-dependent pathologies. A variety of animal models have been described to provide more quantitative analysis of in vivo angiogenesis and to characterize pro- and antiangiogenic molecules. However, it is still necessary to establish a quantitative, reproducible and specific method for studies of angiogenesis factors and inhibitors. This work aimed to standardize a method for the study of angiogenesis and to investigate the effects of thalidomide on angiogenesis. Sponges of 0.5 x 0.5 x 0.5 cm were implanted in the back of mice groups, control and experimental (thalidomide 200 mg/K/day by gavage). After seven days, the sponges were removed. The dosage of hemoglobin in sponge and in circulation was performed and the ratio between the values was tested using nonparametric Mann-Whitney test. Results have shown that sponge-induced angiogenesis quantitated by ratio between hemoglobin content in serum and in sponge is a helpful model for in vivo studies on angiogenesis. Moreover, it was observed that sponge-induced angiogenesis can be suppressed by thalidomide, corroborating to the validity of the standardized method. 2013 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32729375026 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.85 |
| title | Standardization of a method to study angiogenesis in a mouse model |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) thalidomide sponge implant Angiogenesis model hemoglobin measurement |
| url | https://www.redalyc.org/articulo.oa?id=32729375026 |