Characterization of α-actin isoforms in white and red skeletal muscle types of Leporinus macrocephalus (Characiformes, Anostomidae)
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Academia Brasileira de Ciências
2015
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| _version_ | 1876473237369520128 |
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| author | FERNANDA A. ALVES-COSTA |
| author_facet | FERNANDA A. ALVES-COSTA |
| contents | Characterization of α-actin isoforms in white and red skeletal muscle types of Leporinus macrocephalus (Characiformes, Anostomidae) FERNANDA A. ALVES-COSTA MAELI D.P. SILVA ADRIANE P. WASKO Multidisciplinaria (Ciencias Naturales y Exactas) Ser Phe binding site ATP interaction Ala155 substitution Two α-actin genes of the fish Leporinus macrocephalus, referring to white and red muscle tissues, were isolated. Actin isoforms, that mainly differed by a Ser/Ala155 substitution, can have a functional significance related to actin-ATP interaction. An Ala155 residue, as observed in the α-skeletal actin from red muscle, results in a decrease in actin’s affinity for ATP, which may also be associated to the slow contractile performance of this tissue. Furthermore, a Phe/Ile262 substitution at the red muscle actin leads to a hydrophobicity variation at the D-plug of the protein, which could alter its stability. Data on qRTPCR evidenced a significant higher actin mRNA level in white muscle when compared to red muscle (T=105 Mann Whitney; p<0.001). This finding could be related to the energetic demands of the white muscle tissue, with fast contraction fibers and glycolytic metabolism for energy supply. Available data on muscle actins lead to the proposal that white and red α-skeletal actins are genetically and functionally distinguishable in fish species, a feature that is not found in other vertebrate groups. 2015 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32743236013 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.87 |
| format | Artículo científico |
| id | redalyc_32743236013 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Characterization of α-actin isoforms in white and red skeletal muscle types of Leporinus macrocephalus (Characiformes, Anostomidae) FERNANDA A. ALVES-COSTA Multidisciplinaria (Ciencias Naturales y Exactas) Ser Phe binding site ATP interaction Ala155 substitution Characterization of α-actin isoforms in white and red skeletal muscle types of Leporinus macrocephalus (Characiformes, Anostomidae) FERNANDA A. ALVES-COSTA MAELI D.P. SILVA ADRIANE P. WASKO Multidisciplinaria (Ciencias Naturales y Exactas) Ser Phe binding site ATP interaction Ala155 substitution Two α-actin genes of the fish Leporinus macrocephalus, referring to white and red muscle tissues, were isolated. Actin isoforms, that mainly differed by a Ser/Ala155 substitution, can have a functional significance related to actin-ATP interaction. An Ala155 residue, as observed in the α-skeletal actin from red muscle, results in a decrease in actin’s affinity for ATP, which may also be associated to the slow contractile performance of this tissue. Furthermore, a Phe/Ile262 substitution at the red muscle actin leads to a hydrophobicity variation at the D-plug of the protein, which could alter its stability. Data on qRTPCR evidenced a significant higher actin mRNA level in white muscle when compared to red muscle (T=105 Mann Whitney; p<0.001). This finding could be related to the energetic demands of the white muscle tissue, with fast contraction fibers and glycolytic metabolism for energy supply. Available data on muscle actins lead to the proposal that white and red α-skeletal actins are genetically and functionally distinguishable in fish species, a feature that is not found in other vertebrate groups. 2015 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32743236013 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.87 |
| title | Characterization of α-actin isoforms in white and red skeletal muscle types of Leporinus macrocephalus (Characiformes, Anostomidae) |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) Ser Phe binding site ATP interaction Ala155 substitution |
| url | https://www.redalyc.org/articulo.oa?id=32743236013 |