FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum- Solanaceae)

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Autor principal: PATRICIA M. AGUILERA
Formato: Artículo científico
Lenguaje:en
Publicado: Academia Brasileira de Ciências 2016
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author PATRICIA M. AGUILERA
author_facet PATRICIA M. AGUILERA
contents FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum- Solanaceae) PATRICIA M. AGUILERA HUMBERTO J. DEBAT MARISEL A. SCALDAFERRO DARDO A. MARTÍ MAURO GRABIELE Multidisciplinaria (Ciencias Naturales y Exactas) FISH mapping Capsicum 5S rRNA gene derived probe We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32744812010 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.88
format Artículo científico
id redalyc_32744812010
institution Redalyc
language en
publishDate 2016
publisher Academia Brasileira de Ciências
spellingShingle FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum- Solanaceae)
PATRICIA M. AGUILERA
Multidisciplinaria (Ciencias Naturales y Exactas)
FISH
mapping
Capsicum
5S rRNA gene
derived probe
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum- Solanaceae) PATRICIA M. AGUILERA HUMBERTO J. DEBAT MARISEL A. SCALDAFERRO DARDO A. MARTÍ MAURO GRABIELE Multidisciplinaria (Ciencias Naturales y Exactas) FISH mapping Capsicum 5S rRNA gene derived probe We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32744812010 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.88
title FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum- Solanaceae)
topic Multidisciplinaria (Ciencias Naturales y Exactas)
FISH
mapping
Capsicum
5S rRNA gene
derived probe
url https://www.redalyc.org/articulo.oa?id=32744812010