Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells
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| Format: | Artículo científico |
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Academia Brasileira de Ciências
2016
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| _version_ | 1876453602176794624 |
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| author | CHARLENE S.C. GARCIA |
| author_facet | CHARLENE S.C. GARCIA |
| contents | Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells CHARLENE S.C. GARCIA CAROLINE MENTI ANA PAULA F. LAMBERT THIAGO BARCELLOS SIDNEI MOURA CAROLINE CALLONI CÁTIA S. BRANCO MIRIAN SALVADOR MARIANA ROESCH-ELY JOÃO A.P. HENRIQUES Multidisciplinaria (Ciencias Naturales y Exactas) cancer apoptosis Antioxidant chemical compounds Salvia officinalis (L Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl- 1-picrylhydrazyl (DPPH • ) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+ ), catalase (CAT- like ) and superoxide dismutase (SOD- like ) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identification sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia of fi cinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, inflammation and cancer. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32744812024 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.88 |
| format | Artículo científico |
| id | redalyc_32744812024 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells CHARLENE S.C. GARCIA Multidisciplinaria (Ciencias Naturales y Exactas) cancer apoptosis Antioxidant chemical compounds Salvia officinalis (L Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells CHARLENE S.C. GARCIA CAROLINE MENTI ANA PAULA F. LAMBERT THIAGO BARCELLOS SIDNEI MOURA CAROLINE CALLONI CÁTIA S. BRANCO MIRIAN SALVADOR MARIANA ROESCH-ELY JOÃO A.P. HENRIQUES Multidisciplinaria (Ciencias Naturales y Exactas) cancer apoptosis Antioxidant chemical compounds Salvia officinalis (L Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl- 1-picrylhydrazyl (DPPH • ) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+ ), catalase (CAT- like ) and superoxide dismutase (SOD- like ) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identification sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia of fi cinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, inflammation and cancer. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32744812024 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.88 |
| title | Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) cancer apoptosis Antioxidant chemical compounds Salvia officinalis (L |
| url | https://www.redalyc.org/articulo.oa?id=32744812024 |