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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2016
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| Online Access: | https://www.redalyc.org/articulo.oa?id=32746362020 |
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Table of Contents:
- Hippocampal distribution of IL-1B and IL-1RI following lithium-pilocarpine-induced status epilepticus in the developing rat DULCE-MARIELY ÁLVAREZ-CRODA JUAN SANTIAGO-GARCÍA JESÚS S. MEDEL-MATUS JOEL MARTÍNEZ-QUIROZ ANGEL A. PUIG-LAGUNES LUIS BELTRÁN-PARRAZAL MARÍA-LEONOR LÓPEZ-MERAZ Multidisciplinaria (Ciencias Naturales y Exactas) IL 1β 1RI hippocampus Developing rat The contribution of Interleukin-1β (IL-1β) to neuronal injury induced by status epilepticus (SE) in the immature brain remains unclear. The goal of this study was to determine the hippocampal expression of IL-1β and its type 1 receptor (IL-1RI) following SE induced by the lithium-pilocarpine model in fourteen-days-old rat pups; control animals were given an equal volume of saline instead of the convulsant. IL-1β and IL-1RI mRNA hippocampal levels were assessed by qRT-PCR 6 and 24 h after SE or control conditions. IL-1β and IL-1RI expression was detected in the dorsal hippocampus by immunohistochemical procedures; Fluoro-Jade B staining was carried out in parallel sections in order to detect neuronal cell death. IL-1β mRNA expression was increased 6 h following SE, but not at 24 h; however IL-1RI mRNA expression was unaffected when comparing with the control group. IL-1β and IL-1RI immunoreactivity was not detected in control animals. IL-1β and IL-1RI were expressed in the CA1 pyramidal layer, the dentate gyrus granular layer and the hilus 6 h after SE, whereas injured cells were detected 24 h following seizures. Early expression of IL-1β and IL-1RI in the hippocampus could be associated with SE-induced neuronal cell death mechanisms in the developing rat. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746362020 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.88