Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys
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| Format: | Artículo científico |
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Academia Brasileira de Ciências
2016
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| author | NATHALIA A. SILVA |
| author_facet | NATHALIA A. SILVA |
| contents | Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys NATHALIA A. SILVA CLEIDE M.R. ALBUQUERQUE ALINE D. MARINHO TÚLIO D. SILVA HELENA S.A. MONTEIRO MÁRCIA V. SILVA MARIA TEREZA S. CORREIA TICIANA P. PEREIRA ALICE M.C. MARTINS DALGIMAR B. MENEZES RAFAEL M. XIMENES RENÉ D. MARTINS ROBERTA J.B. JORGE ANTONIO G. SILVA NETO Multidisciplinaria (Ciencias Naturales y Exactas) 2D PAGE MDCK venom kidney Scorpions belonging to the Tityus genus are of medical interest in Brazil. Among them, Tityus stigmurusis the main scorpion responsible for stings in the Northeast region. After a sting, the scorpion venom distributes rapidly to the organs, reaching the kidneys quickly. However, there are few studies concerning the renal pathophysiology of scorpion poisoning. In this study, we evaluated the effects of T. stigmurusvenom (TsV) on renal parameters in isolated rat kidneys. Wistar rats (n = 6), weighing 250-300 g, were perfused with Krebs-Henseleit solution containing 6 g/100 mL bovine serum albumin. TsV at 0.3 and 1.0 μg/mL was tested, and the effects on perfusion pressure (PP), renal vascular resistance (RVR), urinary fl ow (UF), glomerular fi ltration rate (GFR), and electrolyte excretion were analyzed. Effects were observed only at TsV concentration of 1.0 μg/mL, which increased PP (controlPP40’ = 92.7 ± 1.95; TsVPP40’ = 182.0 ± 4.70* mmHg, *p < 0.05), RVR (controlRV R40’ = 3.28 ± 0.23 mmHg; TstRV R40’ = 6.76 ± 0.45* mmHg, *p < 0.05), UF (controlUF50’ = 0.16 ± 0.04; TstUF50’ = 0.60 ± 0.10* mL/g/min,*p < 0.05), GFR and electrolyte excretion, with histological changes that indicate renal tubular injury. In conclusion, T. stigmurus venom induces a transient increase in PP with tubular injury, both of which lead to an augmented electrolyte excretion. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746362021 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.88 |
| format | Artículo científico |
| id | redalyc_32746362021 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys NATHALIA A. SILVA Multidisciplinaria (Ciencias Naturales y Exactas) 2D PAGE MDCK venom kidney Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys NATHALIA A. SILVA CLEIDE M.R. ALBUQUERQUE ALINE D. MARINHO TÚLIO D. SILVA HELENA S.A. MONTEIRO MÁRCIA V. SILVA MARIA TEREZA S. CORREIA TICIANA P. PEREIRA ALICE M.C. MARTINS DALGIMAR B. MENEZES RAFAEL M. XIMENES RENÉ D. MARTINS ROBERTA J.B. JORGE ANTONIO G. SILVA NETO Multidisciplinaria (Ciencias Naturales y Exactas) 2D PAGE MDCK venom kidney Scorpions belonging to the Tityus genus are of medical interest in Brazil. Among them, Tityus stigmurusis the main scorpion responsible for stings in the Northeast region. After a sting, the scorpion venom distributes rapidly to the organs, reaching the kidneys quickly. However, there are few studies concerning the renal pathophysiology of scorpion poisoning. In this study, we evaluated the effects of T. stigmurusvenom (TsV) on renal parameters in isolated rat kidneys. Wistar rats (n = 6), weighing 250-300 g, were perfused with Krebs-Henseleit solution containing 6 g/100 mL bovine serum albumin. TsV at 0.3 and 1.0 μg/mL was tested, and the effects on perfusion pressure (PP), renal vascular resistance (RVR), urinary fl ow (UF), glomerular fi ltration rate (GFR), and electrolyte excretion were analyzed. Effects were observed only at TsV concentration of 1.0 μg/mL, which increased PP (controlPP40’ = 92.7 ± 1.95; TsVPP40’ = 182.0 ± 4.70* mmHg, *p < 0.05), RVR (controlRV R40’ = 3.28 ± 0.23 mmHg; TstRV R40’ = 6.76 ± 0.45* mmHg, *p < 0.05), UF (controlUF50’ = 0.16 ± 0.04; TstUF50’ = 0.60 ± 0.10* mL/g/min,*p < 0.05), GFR and electrolyte excretion, with histological changes that indicate renal tubular injury. In conclusion, T. stigmurus venom induces a transient increase in PP with tubular injury, both of which lead to an augmented electrolyte excretion. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746362021 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.88 |
| title | Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) 2D PAGE MDCK venom kidney |
| url | https://www.redalyc.org/articulo.oa?id=32746362021 |