Multivariate optimization of a method for antimony determination by hydride generation atomic fluorescence spectrometry in hair samples of patients undergoing chemotherapy against Leishmaniasis

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Main Author:	Manuelle C. Cardozo
Format:	Artículo científico
Language:	en
Published:	Academia Brasileira de Ciências 2016
Subjects:	Multidisciplinaria (Ciencias Naturales y Exactas) HG AFS hair antimony Leishmaniasis
Online Access:	Acceder al recurso
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author	Manuelle C. Cardozo
author_facet	Manuelle C. Cardozo
contents	Multivariate optimization of a method for antimony determination by hydride generation atomic fluorescence spectrometry in hair samples of patients undergoing chemotherapy against Leishmaniasis Manuelle C. Cardozo Dannuza D. Cavalcante Daniel L.F. Silva Walter N.L. Dos Santos Marcos A. Bezerra Multidisciplinaria (Ciencias Naturales y Exactas) HG AFS hair antimony Leishmaniasis A method was developed for determination of total antimony in hair samples from patients undergoing chemotherapy against Leishmaniasis based on the administration of pentavalent antimonial drugs. The method is based on microwave assisted digestion of the samples in a pressurized system, reduction of Sb 5+ to Sb 3+ with KI solution (10% w/v) in ascorbic acid (2%, w/v) and its subsequent determination by hydride generation atomic fluorescence spectrometry (HG-AFS). The proportions of each component (HCl, HNO 3 and water) used in the digestion were studied applying a constrained mixtures design. The optimal proportions found were 50% water, 25% HNO 3 and 25% HCl. Variables involved in the generation of antimony hydride were optimized using a Doehlert design revealing that good sensitivity is found when using 2.0% w/v NaBH 4 and 4.4 mol L -1 HCl. Under the optimum experimental conditions, the method allows the determination of antimony in hair samples with detection and quantification limits of 1.4 and 4.6 ng g -1 , respectively, and precision expressed as relative standard deviation (RSD) of 2.8% (n = 10 to 10.0 mg L -1 ). The developed method was applied in the analysis of hair samples from patients who take medication against Leishmaniasis. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746972002 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.88
format	Artículo científico
id	redalyc_32746972002
language	en
publishDate	2016
publisher	Academia Brasileira de Ciências
spellingShingle	Multivariate optimization of a method for antimony determination by hydride generation atomic fluorescence spectrometry in hair samples of patients undergoing chemotherapy against Leishmaniasis Manuelle C. Cardozo Multidisciplinaria (Ciencias Naturales y Exactas) HG AFS hair antimony Leishmaniasis Multivariate optimization of a method for antimony determination by hydride generation atomic fluorescence spectrometry in hair samples of patients undergoing chemotherapy against Leishmaniasis Manuelle C. Cardozo Dannuza D. Cavalcante Daniel L.F. Silva Walter N.L. Dos Santos Marcos A. Bezerra Multidisciplinaria (Ciencias Naturales y Exactas) HG AFS hair antimony Leishmaniasis A method was developed for determination of total antimony in hair samples from patients undergoing chemotherapy against Leishmaniasis based on the administration of pentavalent antimonial drugs. The method is based on microwave assisted digestion of the samples in a pressurized system, reduction of Sb 5+ to Sb 3+ with KI solution (10% w/v) in ascorbic acid (2%, w/v) and its subsequent determination by hydride generation atomic fluorescence spectrometry (HG-AFS). The proportions of each component (HCl, HNO 3 and water) used in the digestion were studied applying a constrained mixtures design. The optimal proportions found were 50% water, 25% HNO 3 and 25% HCl. Variables involved in the generation of antimony hydride were optimized using a Doehlert design revealing that good sensitivity is found when using 2.0% w/v NaBH 4 and 4.4 mol L -1 HCl. Under the optimum experimental conditions, the method allows the determination of antimony in hair samples with detection and quantification limits of 1.4 and 4.6 ng g -1 , respectively, and precision expressed as relative standard deviation (RSD) of 2.8% (n = 10 to 10.0 mg L -1 ). The developed method was applied in the analysis of hair samples from patients who take medication against Leishmaniasis. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746972002 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.88
title	Multivariate optimization of a method for antimony determination by hydride generation atomic fluorescence spectrometry in hair samples of patients undergoing chemotherapy against Leishmaniasis
topic	Multidisciplinaria (Ciencias Naturales y Exactas) HG AFS hair antimony Leishmaniasis
url	https://www.redalyc.org/articulo.oa?id=32746972002

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