Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples
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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2016
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| _version_ | 1876469417059024896 |
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| author | Ana Lucia S.M. Felicio |
| author_facet | Ana Lucia S.M. Felicio |
| contents | Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples Ana Lucia S.M. Felicio Alessandra M. Monteiro Mariana B. Almeida Tiago B. Madeira Suzana L. Nixdorf Maria Josefa S. Yabe Multidisciplinaria (Ciencias Naturales y Exactas) UV HPLC soil Pesticides degradation products Diuron is one of the most widely herbicide used worldwide, which can undergo degradation produc- ing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-di- chloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quan- titation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C 18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min − 1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R 2 > 0.99), presenting more than 90% of recovery. The method was successfully applied to quan- tify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746972007 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.88 |
| format | Artículo científico |
| id | redalyc_32746972007 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples Ana Lucia S.M. Felicio Multidisciplinaria (Ciencias Naturales y Exactas) UV HPLC soil Pesticides degradation products Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples Ana Lucia S.M. Felicio Alessandra M. Monteiro Mariana B. Almeida Tiago B. Madeira Suzana L. Nixdorf Maria Josefa S. Yabe Multidisciplinaria (Ciencias Naturales y Exactas) UV HPLC soil Pesticides degradation products Diuron is one of the most widely herbicide used worldwide, which can undergo degradation produc- ing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-di- chloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quan- titation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C 18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min − 1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R 2 > 0.99), presenting more than 90% of recovery. The method was successfully applied to quan- tify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746972007 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.88 |
| title | Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) UV HPLC soil Pesticides degradation products |
| url | https://www.redalyc.org/articulo.oa?id=32746972007 |