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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2016
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| Online Access: | https://www.redalyc.org/articulo.oa?id=32746972021 |
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| _version_ | 1866811658767171584 |
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| author | FRANCISCO R.S. CARVALHO |
| author_facet | FRANCISCO R.S. CARVALHO |
| contents | Are salty liquid food flavorings in vitro antitumor substances? FRANCISCO R.S. CARVALHO ANTONIO G. MOURA GARDENIA F. RODRIGUES NARCIA M.F. NUNES DAISY J.B. LIMA CLAUDIA PESSOA MARCILIA P. COSTA PAULO M.P. FERREIRA ANA PAULA PERON Multidisciplinaria (Ciencias Naturales y Exactas) toxicity Allium cepa Food additives neoplasic cells antiproliferative action The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9- 1500 μg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC 50 values ranging from 125.4 μg/mL (Cheddar - HCT-116) to 402.6 μg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 μg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746972021 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.88 |
| format | Artículo científico |
| id | redalyc_32746972021 |
| language | en |
| publishDate | 2016 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Are salty liquid food flavorings in vitro antitumor substances? FRANCISCO R.S. CARVALHO Multidisciplinaria (Ciencias Naturales y Exactas) toxicity Allium cepa Food additives neoplasic cells antiproliferative action Are salty liquid food flavorings in vitro antitumor substances? FRANCISCO R.S. CARVALHO ANTONIO G. MOURA GARDENIA F. RODRIGUES NARCIA M.F. NUNES DAISY J.B. LIMA CLAUDIA PESSOA MARCILIA P. COSTA PAULO M.P. FERREIRA ANA PAULA PERON Multidisciplinaria (Ciencias Naturales y Exactas) toxicity Allium cepa Food additives neoplasic cells antiproliferative action The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9- 1500 μg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC 50 values ranging from 125.4 μg/mL (Cheddar - HCT-116) to 402.6 μg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 μg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells. 2016 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32746972021 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.88 |
| title | Are salty liquid food flavorings in vitro antitumor substances? |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) toxicity Allium cepa Food additives neoplasic cells antiproliferative action |
| url | https://www.redalyc.org/articulo.oa?id=32746972021 |