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| Format: | Artículo científico |
| Sprache: | en |
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Academia Brasileira de Ciências
2017
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| Online-Zugang: | https://www.redalyc.org/articulo.oa?id=32752675021 |
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| _version_ | 1866811642450280448 |
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| author | ARTURO B. MARTINELLI |
| author_facet | ARTURO B. MARTINELLI |
| contents | The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps ARTURO B. MARTINELLI CECILIA WAICHERT DIEGO N. BARBOSA VALÉRIA FAGUNDES CELSO O. AZEVEDO Multidisciplinaria (Ciencias Naturales y Exactas) KOH Wolbachia Diaphanization genitalia preparation Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA. 2017 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32752675021 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.89 |
| format | Artículo científico |
| id | redalyc_32752675021 |
| language | en |
| publishDate | 2017 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps ARTURO B. MARTINELLI Multidisciplinaria (Ciencias Naturales y Exactas) KOH Wolbachia Diaphanization genitalia preparation The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps ARTURO B. MARTINELLI CECILIA WAICHERT DIEGO N. BARBOSA VALÉRIA FAGUNDES CELSO O. AZEVEDO Multidisciplinaria (Ciencias Naturales y Exactas) KOH Wolbachia Diaphanization genitalia preparation Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA. 2017 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32752675021 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.89 |
| title | The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) KOH Wolbachia Diaphanization genitalia preparation |
| url | https://www.redalyc.org/articulo.oa?id=32752675021 |