Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses

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Main Author: RAIMUNDO DA SILVA
Format: Artículo científico
Language:en
Published: Academia Brasileira de Ciências 2017
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author RAIMUNDO DA SILVA
author_facet RAIMUNDO DA SILVA
contents Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses RAIMUNDO DA SILVA DANILLO SILVA IVANA VENEZA IRACILDA SAMPAIO HORACIO SCHNEIDER GRAZIELLE GOMES Multidisciplinaria (Ciencias Naturales y Exactas) PCR EPIC intron Lutjanus Southern Red Snapper In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater than the intragenic regions commonly used in population genetics studies. Moreover, six out of eight markers showed cross- amplification with other taxa. Thus, the primers described here may be used to elucidate questions at the intraspecific level for a large number of taxa. 2017 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32753602012 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.89
format Artículo científico
id redalyc_32753602012
institution Redalyc
language en
publishDate 2017
publisher Academia Brasileira de Ciências
spellingShingle Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
RAIMUNDO DA SILVA
Multidisciplinaria (Ciencias Naturales y Exactas)
PCR
EPIC
intron
Lutjanus
Southern Red Snapper
Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses RAIMUNDO DA SILVA DANILLO SILVA IVANA VENEZA IRACILDA SAMPAIO HORACIO SCHNEIDER GRAZIELLE GOMES Multidisciplinaria (Ciencias Naturales y Exactas) PCR EPIC intron Lutjanus Southern Red Snapper In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater than the intragenic regions commonly used in population genetics studies. Moreover, six out of eight markers showed cross- amplification with other taxa. Thus, the primers described here may be used to elucidate questions at the intraspecific level for a large number of taxa. 2017 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32753602012 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.89
title Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses
topic Multidisciplinaria (Ciencias Naturales y Exactas)
PCR
EPIC
intron
Lutjanus
Southern Red Snapper
url https://www.redalyc.org/articulo.oa?id=32753602012