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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2000
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| Online Access: | https://www.redalyc.org/articulo.oa?id=32772307 |
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Table of Contents:
- Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis Nilson Tavares Nunes Narcisa Silva Leal Cunha e Aída Voloch Hassón Multidisciplinaria (Ciencias Naturales y Exactas) electrocyte denervation immunohistochemistry choline acetyltransferase Acetylcholine is the neurotransmitter responsible for the transmission of impulses from cholinergic neuronsto cells of innervated tissues. Its biosynthesis is catalyzed by the enzyme Choline acetyltransferase that isconsidered to be a phenotypically specific marker for cholinergic system. It is well known that the regulationof Choline acetyltransferase activity under physiological and pathological conditions is importantfor development and neuronal activities of cholinergic functions. We observed the distribution of Cholineacetyltransferase in sections from the normal and denervated main electric organ sections of Electrophoruselectricus (L.) by immunofluorescence using a anti-Choline acetyltransferase antibody. The animalswere submitted to a surgical procedure to remove about 20 nerves and after 30 and 60 days, they weresacrificed. After 30 days, the results from immunohistochemistry demonstrated an increase on the Cholineacetyltransferase distribution at denervated tissue sections when compared with the sections from the normalcontralateral organ. A very similar labeling was observed between normal and denervated tissue sections ofthe animals after 60 days. However, Choline acetyltransferase activity (nmolesACh/ min/ mg of protein) inextracts obtained from electrocyte microsomal preparation, estimated by Fonnuns method (Fonnun 1975),was 70% lower in the denervated extracts. 2000 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32772307 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.72