Watery and dark axons in wallerian degeneration of the opossum´s optic nerv: different patterns of cytoskeletal breakdown?

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1. Verfasser: Marcelo S. Narciso
Format: Artículo científico
Sprache:en
Veröffentlicht: Academia Brasileira de Ciências 2001
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author Marcelo S. Narciso
author_facet Marcelo S. Narciso
contents Watery and dark axons in wallerian degeneration of the opossum´s optic nerv: different patterns of cytoskeletal breakdown? Marcelo S. Narciso Jan Nora Hokoç Ana M. B. Martinez Multidisciplinaria (Ciencias Naturales y Exactas) marsupial nerve fiber crush lesion ultrastructure dark degeneration In this paper we report a qualitative morphological analysis ofWallerian degeneration in a marsupial. Rightoptic nerves of opossums Didelphis marsupialis were crushed with a fine forceps and after 24, 48, 72, 96and 168 hours the animals were anaesthetized and perfused with fixative. The optic nerves were immersed infixative and processed for routine transmission electron microscopy. Among the early alterations typical ofaxonal degeneration, we observed nerve fibers with focal degeneration of the axoplasmic cytoskeleton, waterydegeneration and dark degeneration, the latter being prevalent at 168 hours after crush. Our results point toa gradual disintegration of the axoplasmic cytoskeleton, opposed to the previous view of an “all-or-nothing”process (Griffin et al 1995). We also report that, due to an unknown mechanism, fibers show either a darkor watery pattern of axonal degeneration, as observed in axon profiles. We also observed fibers undergoingearly myelin breakdown in the absence of axonal alterations. 2001 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32773208 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.2 Vol.73
format Artículo científico
id redalyc_32773208
institution Redalyc
language en
publishDate 2001
publisher Academia Brasileira de Ciências
spellingShingle Watery and dark axons in wallerian degeneration of the opossum´s optic nerv: different patterns of cytoskeletal breakdown?
Marcelo S. Narciso
Multidisciplinaria (Ciencias Naturales y Exactas)
marsupial
nerve fiber
crush lesion
ultrastructure
dark degeneration
Watery and dark axons in wallerian degeneration of the opossum´s optic nerv: different patterns of cytoskeletal breakdown? Marcelo S. Narciso Jan Nora Hokoç Ana M. B. Martinez Multidisciplinaria (Ciencias Naturales y Exactas) marsupial nerve fiber crush lesion ultrastructure dark degeneration In this paper we report a qualitative morphological analysis ofWallerian degeneration in a marsupial. Rightoptic nerves of opossums Didelphis marsupialis were crushed with a fine forceps and after 24, 48, 72, 96and 168 hours the animals were anaesthetized and perfused with fixative. The optic nerves were immersed infixative and processed for routine transmission electron microscopy. Among the early alterations typical ofaxonal degeneration, we observed nerve fibers with focal degeneration of the axoplasmic cytoskeleton, waterydegeneration and dark degeneration, the latter being prevalent at 168 hours after crush. Our results point toa gradual disintegration of the axoplasmic cytoskeleton, opposed to the previous view of an “all-or-nothing”process (Griffin et al 1995). We also report that, due to an unknown mechanism, fibers show either a darkor watery pattern of axonal degeneration, as observed in axon profiles. We also observed fibers undergoingearly myelin breakdown in the absence of axonal alterations. 2001 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32773208 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.2 Vol.73
title Watery and dark axons in wallerian degeneration of the opossum´s optic nerv: different patterns of cytoskeletal breakdown?
topic Multidisciplinaria (Ciencias Naturales y Exactas)
marsupial
nerve fiber
crush lesion
ultrastructure
dark degeneration
url https://www.redalyc.org/articulo.oa?id=32773208