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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2001
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| Online Access: | https://www.redalyc.org/articulo.oa?id=32773409 |
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Table of Contents:
- Embryonic hybrid cells: a powerful tool for studying pluripotency and reprogramming of the differentiated cell chromosomes Oleg Serov Natalia Matveeva Sergey Kuznetsov Elena Kaftanovskaya Josane Mittmann Multidisciplinaria (Ciencias Naturales y Exactas) ES cells EG cells hybrid cells pluripotency reprogramming The properties of embryonic hybrid cells obtained by fusion of embryonic stem (ES) or teratocarcinoma (TC)cells with differentiated cells are reviewed. Usually, ES-somatic or TC-somatic hybrids retain pluripotentcapacity at high levels quite comparable or nearly identical with those of the pluripotent partner. Whencultured in vitro, ES-somatic- and TC-somatic hybrid cell clones, as a rule, lose the chromosomes derivedfrom the somatic partner; however, in some clones the autosomes from theEScell partner were also eliminated,i.e. the parental chromosomes segregated bilaterally in the ES-somatic cell hybrids. This opens up ways forsearching correlation between the pluripotent status of the hybrid cells and chromosome segregation patternsand therefore for identifying the particular chromosomes involved in the maintenance of pluripotency. Useof selective medium allows to isolate in vitro the clones of ES-somatic hybrid cells in which the pluripotentchromosome can be replaced by the somatic counterpart carrying the selectable gene. Unlike the TCsomaticcell hybrids, the ES-somatic hybrids with a near-diploid complement of chromosomes are able tocontribute to various tissues of chimeric animals after injection into the blastocoel cavity. Analysis of thechimeric animals showed that the somatic chromosome undergoes reprogramming during development.The prospects for the identification of the chromosomes that are involved in the maintenance of pluripotencyand its cis- and trans-regulation in the hybrid cell genome are discussed. 2001 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32773409 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.73