Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle
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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2004
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| _version_ | 1876448654776074240 |
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| author | Mauro Sola-Penna |
| author_facet | Mauro Sola-Penna |
| contents | Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle Mauro Sola-Penna Ana Paula P. Silva Gutemberg G. Alves Alexandre H.B. Araújo Multidisciplinaria (Ciencias Naturales y Exactas) actin rabbit hormone metabolism glycolysis In this work, we report evidences that the association of phosphofructokinase and F-actin can be affected by insulin stimulation in rabbit skeletal muscle homogenates and that this association can be a mechanism of phos-phofructokinase regulation. Through co-sedimentation techniques, we observed that on insulin-stimulated tissues, approximately 70% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 28% in control. This phenomenon is accompanied by a 100% increase in specific phosphofructokinase activity in stimulated homogenates. Purified F-actin causes an increase of 230% in phosphofructokinase activity and alters its kinetic parameters. The presence of F-actin increases the affinity of phosphofructokinase for fructose 6-phosphate nevertheless, with no changes in maximum velocity (Vmax). Here we propose that the modulation of cellular distribution of phosphofructokinase may be one of the mechanisms of control of glycolytic flux in mammalian muscle by insulin. 2004 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32776308 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.76 |
| format | Artículo científico |
| id | redalyc_32776308 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle Mauro Sola-Penna Multidisciplinaria (Ciencias Naturales y Exactas) actin rabbit hormone metabolism glycolysis Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle Mauro Sola-Penna Ana Paula P. Silva Gutemberg G. Alves Alexandre H.B. Araújo Multidisciplinaria (Ciencias Naturales y Exactas) actin rabbit hormone metabolism glycolysis In this work, we report evidences that the association of phosphofructokinase and F-actin can be affected by insulin stimulation in rabbit skeletal muscle homogenates and that this association can be a mechanism of phos-phofructokinase regulation. Through co-sedimentation techniques, we observed that on insulin-stimulated tissues, approximately 70% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 28% in control. This phenomenon is accompanied by a 100% increase in specific phosphofructokinase activity in stimulated homogenates. Purified F-actin causes an increase of 230% in phosphofructokinase activity and alters its kinetic parameters. The presence of F-actin increases the affinity of phosphofructokinase for fructose 6-phosphate nevertheless, with no changes in maximum velocity (Vmax). Here we propose that the modulation of cellular distribution of phosphofructokinase may be one of the mechanisms of control of glycolytic flux in mammalian muscle by insulin. 2004 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32776308 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.76 |
| title | Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) actin rabbit hormone metabolism glycolysis |
| url | https://www.redalyc.org/articulo.oa?id=32776308 |