Gap junctions in hematopoietic stroma control proliferation and differentiation of blood cell precursors
Fuente:
Redalyc
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Academia Brasileira de Ciências
2004
|
| Schlagworte: | |
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1876433334413819904 |
|---|---|
| author | Estevão Bodi |
| author_facet | Estevão Bodi |
| contents | Gap junctions in hematopoietic stroma control proliferation and differentiation of blood cell precursors Estevão Bodi Sandra P. Hurtado Marcelo A. Carvalho Radovan Borojevic Antônio C. Carvalho Multidisciplinaria (Ciencias Naturales y Exactas) 17 cells connexins bone marrow hematopoiesis Gap junctions We examined gap junction communication in an in vitro model of hematopoiesis, using the murine bonemarrowstroma cell line S-17, and primary cultures of murine marrow-derived blood cell precursors. S-17 cellsexpress several connexins, the major one being connexin 43. Connexin expression and formation of functionalgap junctions is modulated by stroma cell density. Transfection of S-17 cells with a vector containing connexin43 sense or anti-sense sequences increased or decreased, respectively, connexin 43 synthesis and intercellulardye coupling. Under these conditions, modulation of gap junction-mediated communication modified thegrowth pattern of stroma itself, as well as the ability of the stroma to sustain hematopoiesis. Increasedconnexin 43 expression was associated with a delay in differentiation of blood cells, resulting in increasedproduction of hematopoietic precursors, while decreased connexin 43 expression elicited an accelerateddifferentiation of myeloid blood cell precursor cells. These results suggest that connexin-mediated couplingin the stroma modulates the ratio between proliferation and differentiation of hematopoietic precursors. Wetherefore propose that increased gap junction communication in the stroma elicits an enhanced productionof immature bone marrow cells through the delay in their terminal differentiation, inducing consequently anextended proliferation period of blood cell precursors. 2004 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32776409 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.76 |
| format | Artículo científico |
| id | redalyc_32776409 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Gap junctions in hematopoietic stroma control proliferation and differentiation of blood cell precursors Estevão Bodi Multidisciplinaria (Ciencias Naturales y Exactas) 17 cells connexins bone marrow hematopoiesis Gap junctions Gap junctions in hematopoietic stroma control proliferation and differentiation of blood cell precursors Estevão Bodi Sandra P. Hurtado Marcelo A. Carvalho Radovan Borojevic Antônio C. Carvalho Multidisciplinaria (Ciencias Naturales y Exactas) 17 cells connexins bone marrow hematopoiesis Gap junctions We examined gap junction communication in an in vitro model of hematopoiesis, using the murine bonemarrowstroma cell line S-17, and primary cultures of murine marrow-derived blood cell precursors. S-17 cellsexpress several connexins, the major one being connexin 43. Connexin expression and formation of functionalgap junctions is modulated by stroma cell density. Transfection of S-17 cells with a vector containing connexin43 sense or anti-sense sequences increased or decreased, respectively, connexin 43 synthesis and intercellulardye coupling. Under these conditions, modulation of gap junction-mediated communication modified thegrowth pattern of stroma itself, as well as the ability of the stroma to sustain hematopoiesis. Increasedconnexin 43 expression was associated with a delay in differentiation of blood cells, resulting in increasedproduction of hematopoietic precursors, while decreased connexin 43 expression elicited an accelerateddifferentiation of myeloid blood cell precursor cells. These results suggest that connexin-mediated couplingin the stroma modulates the ratio between proliferation and differentiation of hematopoietic precursors. Wetherefore propose that increased gap junction communication in the stroma elicits an enhanced productionof immature bone marrow cells through the delay in their terminal differentiation, inducing consequently anextended proliferation period of blood cell precursors. 2004 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32776409 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.76 |
| title | Gap junctions in hematopoietic stroma control proliferation and differentiation of blood cell precursors |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) 17 cells connexins bone marrow hematopoiesis Gap junctions |
| url | https://www.redalyc.org/articulo.oa?id=32776409 |