Molecular basis of mammalian cell invasion by Trypanosoma cruzi

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Main Author: Nobuko Yoshida
Format: Artículo científico
Language:en
Published: Academia Brasileira de Ciências 2006
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author Nobuko Yoshida
author_facet Nobuko Yoshida
contents Molecular basis of mammalian cell invasion by Trypanosoma cruzi Nobuko Yoshida Multidisciplinaria (Ciencias Naturales y Exactas) cell invasion trypomastigotes Trypanosoma cruzi Ca2+ mobilization signal transduction Establishment of infection by Trypanosoma cruzi, the agent of Chagas´ disease, depends on a series of eventsinvolving interactions of diverse parasite molecules with host components. Here we focus on the mechanismsof target cell invasion by metacyclic trypomastigotes (MT) and mammalian tissue culture trypomastigotes(TCT). During MT or TCT internalization, signal transduction pathways are activated both in the parasiteand the target cell, leading to Ca2+ mobilization. For cell adhesion, MT engage surface glycoproteins, suchas gp82 and gp35/50, which are Ca2+ signal-inducing molecules. In T. cruzi isolates that enter host cells ingp82-mediated manner, parasite protein tyrosine kinase as well as phospholipase C are activated, and Ca2+is released from IP3-sensitive stores, whereas in T. cruzi isolates that attach to target cells mainly throughgp35/50, the signaling pathway involving adenylate cyclase appears to be stimulated, with Ca2+ release fromacidocalciosomes. In addition, T. cruzi isolate-dependent inhibitory signals, mediated by MT-specific gp90,may be triggered both in the host cell and the parasite. The repertoire of TCT molecules implicated in cellinvasion includes surface glycoproteins of gp85 family, with members containing binding sites for lamininand cytokeratin 18, enzymes such as cruzipain, trans-sialidase, and an oligopeptidase B that generates aCa2+-agonist from a precursor molecule. 2006 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32778110 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.78
format Artículo científico
id redalyc_32778110
institution Redalyc
language en
publishDate 2006
publisher Academia Brasileira de Ciências
spellingShingle Molecular basis of mammalian cell invasion by Trypanosoma cruzi
Nobuko Yoshida
Multidisciplinaria (Ciencias Naturales y Exactas)
cell invasion
trypomastigotes
Trypanosoma cruzi
Ca2+ mobilization
signal transduction
Molecular basis of mammalian cell invasion by Trypanosoma cruzi Nobuko Yoshida Multidisciplinaria (Ciencias Naturales y Exactas) cell invasion trypomastigotes Trypanosoma cruzi Ca2+ mobilization signal transduction Establishment of infection by Trypanosoma cruzi, the agent of Chagas´ disease, depends on a series of eventsinvolving interactions of diverse parasite molecules with host components. Here we focus on the mechanismsof target cell invasion by metacyclic trypomastigotes (MT) and mammalian tissue culture trypomastigotes(TCT). During MT or TCT internalization, signal transduction pathways are activated both in the parasiteand the target cell, leading to Ca2+ mobilization. For cell adhesion, MT engage surface glycoproteins, suchas gp82 and gp35/50, which are Ca2+ signal-inducing molecules. In T. cruzi isolates that enter host cells ingp82-mediated manner, parasite protein tyrosine kinase as well as phospholipase C are activated, and Ca2+is released from IP3-sensitive stores, whereas in T. cruzi isolates that attach to target cells mainly throughgp35/50, the signaling pathway involving adenylate cyclase appears to be stimulated, with Ca2+ release fromacidocalciosomes. In addition, T. cruzi isolate-dependent inhibitory signals, mediated by MT-specific gp90,may be triggered both in the host cell and the parasite. The repertoire of TCT molecules implicated in cellinvasion includes surface glycoproteins of gp85 family, with members containing binding sites for lamininand cytokeratin 18, enzymes such as cruzipain, trans-sialidase, and an oligopeptidase B that generates aCa2+-agonist from a precursor molecule. 2006 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32778110 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.78
title Molecular basis of mammalian cell invasion by Trypanosoma cruzi
topic Multidisciplinaria (Ciencias Naturales y Exactas)
cell invasion
trypomastigotes
Trypanosoma cruzi
Ca2+ mobilization
signal transduction
url https://www.redalyc.org/articulo.oa?id=32778110