Acidification of the parasitophorous vacuole containing Toxoplasma gondii in the presence of hydroxyurea
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| Natura: | Artículo científico |
| Lingua: | en |
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Academia Brasileira de Ciências
2006
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| _version_ | 1876460746194288640 |
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| author | Cristiane S. Carvalho |
| author_facet | Cristiane S. Carvalho |
| contents | Acidification of the parasitophorous vacuole containing Toxoplasma gondii in the presence of hydroxyurea Cristiane S. Carvalho Edésio J. T. Melo Multidisciplinaria (Ciencias Naturales y Exactas) Hydroxyurea Toxoplasma gondii parasitophorous vacuole Toxoplasma gondii multiplies within parasitophorous vacuole that is not recognized by the primary nooxidative defense of host cells, mainly represented by the fusion with acidic organelles. Recent studieshave already shown that hydroxyurea arrested the intracellular parasites leading to its destruction. In thepresent work we investigated the cellular mechanism involved in the destruction of intracellular Toxoplasmagondii. Fluorescent vital stains were used in order to observe possible acidification of parasitophorousvacuole-containing Toxoplasma gondii in presence of hydroxyurea. Vero cells infected with tachyzoiteswere treated with hydroxyurea for 12, 24 or 48 hours. Fluorescence, indicative of acidification, was observedin the parasitophorous vacuole when the cultures were incubated in presence of acridine orange. LysoTrackerred was used in order to determine whether lysosomes were involved in the acidification process. An intensefluorescence was observed after 12 and 24 hours of incubation with hydroxyurea, achieving it is highlyintensity after 48 hours of treatment. Ultrastructural cytochemistry for localization of the acid phosphataselysosomal enzyme was realized. Treated infected cultures showed reaction product in vesicles fusing withvacuole or associated with intravacuolar parasites. These results suggest that fusion with lysosomes andacidification of parasitophorous vacuole leads to parasites destruction in the presence pf hydroxyurea. 2006 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32778308 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.78 |
| format | Artículo científico |
| id | redalyc_32778308 |
| institution | Redalyc |
| language | en |
| publishDate | 2006 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Acidification of the parasitophorous vacuole containing Toxoplasma gondii in the presence of hydroxyurea Cristiane S. Carvalho Multidisciplinaria (Ciencias Naturales y Exactas) Hydroxyurea Toxoplasma gondii parasitophorous vacuole Acidification of the parasitophorous vacuole containing Toxoplasma gondii in the presence of hydroxyurea Cristiane S. Carvalho Edésio J. T. Melo Multidisciplinaria (Ciencias Naturales y Exactas) Hydroxyurea Toxoplasma gondii parasitophorous vacuole Toxoplasma gondii multiplies within parasitophorous vacuole that is not recognized by the primary nooxidative defense of host cells, mainly represented by the fusion with acidic organelles. Recent studieshave already shown that hydroxyurea arrested the intracellular parasites leading to its destruction. In thepresent work we investigated the cellular mechanism involved in the destruction of intracellular Toxoplasmagondii. Fluorescent vital stains were used in order to observe possible acidification of parasitophorousvacuole-containing Toxoplasma gondii in presence of hydroxyurea. Vero cells infected with tachyzoiteswere treated with hydroxyurea for 12, 24 or 48 hours. Fluorescence, indicative of acidification, was observedin the parasitophorous vacuole when the cultures were incubated in presence of acridine orange. LysoTrackerred was used in order to determine whether lysosomes were involved in the acidification process. An intensefluorescence was observed after 12 and 24 hours of incubation with hydroxyurea, achieving it is highlyintensity after 48 hours of treatment. Ultrastructural cytochemistry for localization of the acid phosphataselysosomal enzyme was realized. Treated infected cultures showed reaction product in vesicles fusing withvacuole or associated with intravacuolar parasites. These results suggest that fusion with lysosomes andacidification of parasitophorous vacuole leads to parasites destruction in the presence pf hydroxyurea. 2006 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32778308 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.78 |
| title | Acidification of the parasitophorous vacuole containing Toxoplasma gondii in the presence of hydroxyurea |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) Hydroxyurea Toxoplasma gondii parasitophorous vacuole |
| url | https://www.redalyc.org/articulo.oa?id=32778308 |