Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate
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| Format: | Artículo científico |
| Language: | en |
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Academia Brasileira de Ciências
2006
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| _version_ | 1866812391666221056 |
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| author | Andréa M. Almeida |
| author_facet | Andréa M. Almeida |
| contents | Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate Andréa M. Almeida Clélia R. A. Bertoncini Jiri Borecký Nadja C. Souza-Pinto Aníbal E. Vercesi Multidisciplinaria (Ciencias Naturales y Exactas) iron mtDNA mitochondria oxidative stress lipid peroxidation Iron imbalance/accumulation has been implicated in oxidative injury associated with many degenerativediseases such as hereditary hemochromatosis, β-thalassemia, and Friedreichs ataxia. Mitochondria areparticularly sensitive to iron-induced oxidative stress high loads of iron cause extensive lipid peroxidationand membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrialDNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weightcomplex. IntenseDNAfragmentationwas induced after the incubation of mitochondria with the iron complex.The detection of 3´ phosphoglycolate ends at the mtDNAstrand breaks by a 32P-postlabeling assay, suggestedthe involvement of hydroxyl radical in the DNA fragmentation induced by Fe2+-citrate. Increased levelsof 8-oxo-7,8-dihydro-2´-deoxyguanosine also suggested that Fe2+-citrate-induced oxidative stress causesmitochondrial DNA damage. In conclusion, our results show that iron-mediated lipid peroxidation wasassociated with intense mtDNA damage derived from the direct attack of reactive oxygen species. 2006 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32778310 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.78 |
| format | Artículo científico |
| id | redalyc_32778310 |
| language | en |
| publishDate | 2006 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate Andréa M. Almeida Multidisciplinaria (Ciencias Naturales y Exactas) iron mtDNA mitochondria oxidative stress lipid peroxidation Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate Andréa M. Almeida Clélia R. A. Bertoncini Jiri Borecký Nadja C. Souza-Pinto Aníbal E. Vercesi Multidisciplinaria (Ciencias Naturales y Exactas) iron mtDNA mitochondria oxidative stress lipid peroxidation Iron imbalance/accumulation has been implicated in oxidative injury associated with many degenerativediseases such as hereditary hemochromatosis, β-thalassemia, and Friedreichs ataxia. Mitochondria areparticularly sensitive to iron-induced oxidative stress high loads of iron cause extensive lipid peroxidationand membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrialDNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weightcomplex. IntenseDNAfragmentationwas induced after the incubation of mitochondria with the iron complex.The detection of 3´ phosphoglycolate ends at the mtDNAstrand breaks by a 32P-postlabeling assay, suggestedthe involvement of hydroxyl radical in the DNA fragmentation induced by Fe2+-citrate. Increased levelsof 8-oxo-7,8-dihydro-2´-deoxyguanosine also suggested that Fe2+-citrate-induced oxidative stress causesmitochondrial DNA damage. In conclusion, our results show that iron-mediated lipid peroxidation wasassociated with intense mtDNA damage derived from the direct attack of reactive oxygen species. 2006 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32778310 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.3 Vol.78 |
| title | Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) iron mtDNA mitochondria oxidative stress lipid peroxidation |
| url | https://www.redalyc.org/articulo.oa?id=32778310 |