Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus
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| Format: | Artículo científico |
| Langue: | en |
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Academia Brasileira de Ciências
2007
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| author | Georgia C. Atella |
| author_facet | Georgia C. Atella |
| contents | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus Georgia C. Atella Monica M. Marinho-Carvalho Mario A.C. Silva-Neto Mauro Sola-Penna Gutemberg G. Alves Multidisciplinaria (Ciencias Naturales y Exactas) insect metabolism phosphofructokinase 6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect whichis usually a poor flyer, was measured and compared in two metabolically active tissues flight muscle and fat body.The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg)than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, asmeasured by western-blotting. Muscle extracts are more responsible than fat body toATP and fructose 6-phosphate, bothsubstrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphatepresented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, andcitrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activityfor each tissue, with regulatory patterns that are consistent with their physiological roles. 2007 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32779108 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.79 |
| format | Artículo científico |
| id | redalyc_32779108 |
| institution | Redalyc |
| language | en |
| publishDate | 2007 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus Georgia C. Atella Multidisciplinaria (Ciencias Naturales y Exactas) insect metabolism phosphofructokinase Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus Georgia C. Atella Monica M. Marinho-Carvalho Mario A.C. Silva-Neto Mauro Sola-Penna Gutemberg G. Alves Multidisciplinaria (Ciencias Naturales y Exactas) insect metabolism phosphofructokinase 6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect whichis usually a poor flyer, was measured and compared in two metabolically active tissues flight muscle and fat body.The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg)than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, asmeasured by western-blotting. Muscle extracts are more responsible than fat body toATP and fructose 6-phosphate, bothsubstrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphatepresented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, andcitrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activityfor each tissue, with regulatory patterns that are consistent with their physiological roles. 2007 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32779108 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.79 |
| title | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) insect metabolism phosphofructokinase |
| url | https://www.redalyc.org/articulo.oa?id=32779108 |